The DYS1 Gene Microdeletion And The Sexual Hormone Change Of Idiopathic Male Infertilities

Objective To investigate the microdeletion of DYS1 gene in the long arm of Y chromosome of idiopathic male infertilities. And to investigate the testis pathology and the serum sexual hormone change of male infertilities with DYS1 gene microdeletion.To analysis the change of serum sexual hormone level of idiopathic male infertilities.Method There were sixty idiopathic male infertilities in the experiment group and eighty healthy males in the control group.DYS1 gene was amplified by PCR method. The testis biopsies were embeded in paraffin, stained with H-E, observed under microscope. The serum sexual hormone was test by competitive immunassay method.To analysis the results by SPSS 10.0 software pocket.Result There are eight patients with DYS1 gene microdeletion in the experiment group, none in the control group. The deletion ratio of DYS1 gene is 13.33%(8/60) in the experiment group. The ratio of microdeletion of DYS1 gene is statistic significant between the experiment group and the control group( *2=8.97, P<0.01). The microdeletion of DYS1 gene has relationship with male infertility (0^=13.76). The damage of testis of DYS1 gene microdeletion's varies. There is no sole pathologic type showing.The mean of serum E2 of experiment group is higher than that of control group (t=8.93, P<0.01), the mean of serum T/E2 of experiment group is lower (t= -8. 07, P<0.01).Conclusion The microdeletion of DYS1 gene has relationship with male infertility. The DYS1 gene is a candidate gene for AZF gene.The serum E2 is higher and serum T/E2 lower in idiopathic male infertility, this may be another character of male infertility. It is another evidence to cure male infertility with antiestrogen treatment.