Dynamic Investigation Of CD26/CD30 Expression On T Lymphocytes In Abdominal Infected And Septic Mice

Sepsis with infra-abdominal infection and ensuing multiple organ dysfunction syndrome with septic shock continue to be clinical critical diseases. During different phase of sepsis development, the bias of Th1/ Th2 paradigm may be the critical cause which influences the sepsis prognosis. If a precise immune monitoring can be given , the choose of an appropriate opportunity for immune regulation may promote the clinical survival of sepsis. Dynamic bias of Th1/ Th2 in peripheral blood lymphocytes of abdominal infected and septic mice was investigated on two following aspects in a murine model of sepsis induced by cecal ligation and puncture (CLP), providing evidence for promoting clinical survival of sepsis patients.Animal models were built and divided into CLP and Sham groups. Mortality and pathological changes of lung, liver, kidney and small intestine were observed at 7h, 14h, 26h, 38h, 62h, 86h, 110h, 134h, and 158h after operation. It was shown that animals in Sham groups were all survival, while the highest mortality probability of animals in CLP groups was observed during 14-38h with severe inflammatory pathological changes on each observed organ at 38h after CLP.CD4+ T cell were determined by flow cytometry at 7h, 14h, 26h, 38h, 62h, 86h, 110h, 134h, 158h after operation, CD3+CD4+CD26+(Th1) cells and CD3+CD4+CD30+ (Th2) cell were detected by counting percentage among CD4+ T cell in order to determine the differentiation of Thl and Th2 cell as well as the bias of Th1/Th2. There was higher CD3+CD4+CD26+T cell differentiation in CLP group at 7h after operation than that in control group. The highest and lowest differentiation of CD3+CD4+CD26+ T cell was observed at 14h and 38h after CLP respectively, then it remained on a low level with the SHAM. CD3+CD4+CD30+ T cell differentiation was also increased at 7h after operation and the peak was observed at 38h, which matched with the lowest differentiation of CD3+CD4+CD26+T cell and the highest mortality probability.Taken together, our data indicated that during the initial pathological phase of sepsis (7-14h), immune system was dominated by Thl response and then (26-38h)shifted to Th2 with the development of sepsis. The bias of Th1/2 from Th1 to Th2 andsubsequently cell immune suppression may lead severity and death.