| At present, the treatment of cerebrovascular diseases, paticular ischemic , has not been an efficacious remedy. Thrombolysis therapy can recover cerebral blood stream earlier, ameliorate the blood supply of ischemic penumbra, decrease infarct area and save the brain tissue without necrosis and function, but injuries are inevitable with revascu-larization. The study aims at observating the effects of ultra short wave therapy on brain edema, infarct volume, antioxidant enzyme and free radical after the middle cerebral artery occlusion and reperfusion and providing theroy evidence clinically by utilizing modified Zea - Longafc method to duplicate the rat model.Materials and MethodsFocal cerebral ischemia in rats was produced by 2h occlusion of the middle cerebral artery (MCAO) and 24h reperfusion . Neurological deficits were evaluated by 0 ~ 4 scales ( To select rats). The cerebral water content was. determined by wet/dry weighing method . The infarct volume was measured by 2,3,5 - triphenyltetrazolium chloride (TTC) staining technique. The activity of superoxide dismutase( SOD) and the content of malondialdehyde( MDA) in ischemic tissue of brain and morphology changing were analyzed.ResultsThe decrease in water content and MDA content and relieving hi pathologic injuries as well as the increase in the activity of SOD were significant by ultra short wave therapy on rats when the MCAO was performed for 2h and reperfused for 6h. The variety of infarct volume is not significant.DiscussionNow is considered that a few pathopoiesia mechanisms result in cerebral ischemia reperfusion injuries. Among those ,free radicals are the most important . Free radicals increasing rapidly can react with un-saturated fatty acid on cell membrane after focal ischemia reperfusion, which form superoxide lipid , damage membrane , lead to cell breaking, destroy blood brain barrier and form cerebral edema. MDA is a kind of metabolism product of lipid superoxidation reaction, whose content chang reflects indirectly that of free radicals in the tissue . Moreover ,the more do free radicals produce ,the more antioxidants ,such as SOD,consume. The study indicates brain water content and MDA increased and the activity of SOD decreased when the MCAO was performed for 2h and reperfused for 24h , which means free radicals injuries. Compare to control group , ischemic brain water content and MDA decrease( P < 0.05 ) and SOD increases apparently( P < 0.05 ) by ultra short wave therapy.Ultra short wave has heat and nonheat effect on human. It hasnt been known the mechanism of nonheat effect. In the foretest, we measured interior and surface temperature in the therapeutic area of dead and thermal balance rats with semiconductor point thermometer and infra-red thermometry, then we found the temperature didnt heighten after therapy. The result indicates that the therapeutic dose of the study is in the range of nonheat or little dose. It has been reported that ultra short wave therapy can abatement the water content of trauma cerebral edema experimentally and protect the limb ischemia reperfusion injuries by decreasing free radicals content and increasing SOD content. The study adopts ultra short wave of nonheat dose to treat ischemic rats'head. We found that brain water content and MDA decreased and the activity of SOD increased by ultra short wave therapy on rats when the MCAO was performed for 2h and reperfused for 6h. Little dose of ultra short wave has a few kinds of therapeutic mechanism on human . It is also a heated question that ultra short wave may excite Vagus nerve to make blood vessel expand and blood stream speed up. The study on the time of brain blood stream after MCAO indicates : ischemic brain blood stream increases suddenly , then forms " so call" high irrigating situation in the initial stage ; after reperfusion for 6h , bloodastream decreases and forms low irrigating situation; brain blood stream will stabilize 72h later. So it may have protective effects by relieving low irrigation situation, decreasing... |
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