The Protection Functions Of Betopic Liquid For Retinal Ganglion Cell Of Rabbit Acute Ocular Hypertension Model

ObjectiveTo treat rabbits eyes after acute ocular pressure with Betopic liquid, apoptosis in retinal ganglion cells being tested and cells'ultra-structure being observed, and understand the protection functions of Betopic eye liquid for glaucoma retinal ganglion cell.Methods12 Big ear rabbits were divided randomly into 2 groups: group A which were treated and group B which were not treated. In group A one eye of each rabbit was selected to be made a high ocular pressure model, it was group A2, the other one was control group A1. In group B, the acute ocular pressure group was B2, the other eye group was B1. Rabbit were weighed and anaesthetized and placed in a stereotaxic frame. The anterior chamber of one eye was cannulated with a injector needle through the limbus, which was connected a bottle of NS. To lift the bottle to 136 cm from the rabbit on a vertical distance which can cause a 100mmHg pressure, and maintain 60 mins. 2 hours before elevation of the ocular pressure in the anterior chamber , both eyes of rabbits in the group B were treated with 2 drops Betopic, 2 drops per 12 hours, lasting 3 days. Group A no treating. After 3 days, rabbitswere killed and apoptosis in retinal ganglion cells were tested ( with TUNEL technique) and cells'ultrastmcture were observed with electron microscope. To take count of the number of thapoptosis cells in every microscope view, and the data was treated with statistical calculation , then compare the difference in the four groups.ResultsThe apoptosis cells can be observed in group A2 and group B2,not in group A1 and B1. The apoptosis cells are colorated brown, mainly in inner granule layer and ganglion cells layer. The data was calculated in statistical statistics, the result is; The number of apoptosis in retinal ganglion cells decreases in this sequence: A2, B2, A1 and B1. (P<0. 05). As Electron microscope display, the ultrastruc-ture in group A1 and B1 is normal; A large, dual membrane -bound, usually spherical nucleus is in retinal ganglion cell, plenty of endo-plasmic reticulum ( ER) in protoplast, and mitochondrial crista is clear. In group B2, partial mitochondrial crista swell, vacuolar degeneration and endoplasmic reticulum(ER) dilate partially. In group A2 most mitochondrion are vacuolar degeneration, membranes break, ER dilate.ConclusionHigh ocular pressure can cause retinal ganglion cells apoptosis, while Betopic liquid can partially prevent apoptosis, and Betopic liquid has no toxicity to the retinal nerve cells.