Exogenous Endothelin-1 Injures Cultured Primary Cardiomyocytes From Neonatal Rats

Object To study the effects of exogenous endothelin-1 on cultured primary cardio-myocytes from neonatal rats.Methods Neonatal rat ventricular myocytes were isolated and cultured on 24-well plates. When they grew to a single layer or partly mixed together, we divided wells into ET-1 groups and control groups randomly. Three doses of ET-1 (10-9,10-8 ,10-7mol/L) and two time points (12 hours and 24 hours ) were examined. Morphological changes were observed by a microtelescope, and the levels of LDH ..MDA and SOD in the supernate were examined by physiochemical techniques.Results (1)The morphological and beating characters of cardiomyocytes changed in ET-1 subjects compared to those in the control groups. (2)The levels of LDH and MDA in the supernate increased dose-dependently in ET-1 subjects compared to those in the control groups, significantly (P<0. 05). (3)The levels of SOD in the supernate decreased dose-dependently in ET-1 subjects compared to those in the control groups, significantly (P< 0. 05). (4)The levels of MDA in the supernate increased dose-dependently in ET-1 subjects compared to those in the control groups, significantly (P<0. 05)Conclusion Exogenous endothelin -1 can injure cultured primary cardiomyocytes from neonatal rats, causing cellular structure and metabolic abnormality.