| BackgroundIn recent years, the incidence of malignant lymphoproliferative disease isincreasing. However, there is some difficulty in diagnosing, evaluating prognosis and determining whether they need to keep on being treated and how long they need to keep on being treated. Therefore, it is more important to find a quick, peculiar and sensitive assay in clinical application for assessing malignant lymphoproliferative disease.The genes of antigen acceptors (Ig and TCR) of lymphocytes become functional genes by undergoing rearrangements from germ-line cells to mature cells. The rearrangement genes of antigen acceptors of every lymphocyte are completely different. During the differentiation of a lymphocytes, if a lymphocyte is transformed into malignant cell, then this cell proliferates monoclonally and it will form the malignant disease. Genes of antigen acceptors of normal persons and the patients with lymphadenitis are amplified by PCR and smear phenomenon is observed after electrophoresis because of the different length of the genes. However, the genes of patients with malignant disease are amplified by PCR and a clear band is detected (some are two bands) after electrophoresis because of monoclonal proliferation. Therefore detecting gene rearrangement can distinguish malignant disease from benign disease and diagnose some knotty cases correctly.The complete response rate and recmission time of lymphoproliferative disease are increasing following the improvement of treament nowadays. It is the key to prevent recurrence. The major cause is that there are some residual malignant cells after complete response. It is called minimal residual disease(MRD) because thesemalignant cells cannot be found by morphological means.One malignant cell can be differentiated from 106 normal cells by PCR. Therefore detecting minimal residual disease can mornitor efficacy, evaluate prognosis and guide clinical treatment.This thesis probes the effect of gene rearrangement on lymphoproliferative disease by studying the gene rearrangement of the heavy chain of immunoglobulin (IgH) and T cell receptor- γ (TCR- γ) , which can provide the theoretical basis and the value of clinical utilization in diagnosing malignant lymphoproliferative disease, monitoring the efficacy, evaluating prognosis and guiding treatment.Materials and MethodsSpecimens and tissues1.The specimens are collected from peripheral blood of 32 patients with Non-Hodgkin lymphoma and 8 patients with acute lymphocytic leukemia, bone marrow of 31 patients with Non-Hodgkin lymphoma and wax-embeded tissues of 3 patients with Non-Hodgkin lymphoma. These patients came from the oncology department and blood department of the First Affiliated Hospital of Zhenzhou University. In addition, the peripheral blood of one normal patient was taken for control.2. Wax-embeded tissues of 15 non-Hodgkin lymphoma, 22 lymphadenitis and 14 lymph node tuberculosis were collected from several pathological departments in Henan province. They were diagnosed only by morphology from 1996 to 1998.3. Four specimens of wax-embeded tissue of knotty cases, which could not be diagnosed through consultation of several pathological departments, were collected from the oncology department of the First Affiliated Hospital of Zhenzhou University.4. Five specimens of peripheral blood of NHL patients before and after chemotherapy were collected from the oncology department of the First Affiliated Hospital of Zhengzhou University.MethodThe gene rearrangement was mornitored by polymerase chain reaction including semi-nest PCR and multi-primers PCR ,and then the products of PCR were electrophoresed through 10% polyacrylamide and then the gels were stained with sliver.StatisticalThe experimental data was analyzed with chi-square test. If T is lesser than 1 orn is less than 40, exact probilities in 2*2tables is used.There was a statistical significance when P<0.05 occurred.The Technological Route Wax-embeded tissue Peripheral blood Bone marrow...
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