The Effect Of Acupuncture, Moxibustion And Chinese Herbs On Bcl-2, Bax And PCNA In Cancer Cell Of S180 Model Mice

Objective:Based on the previous clinical and experimental studies, this research deals with the mechanism that acupuncture ,moxibustion and Chinese herbs promote apoptosis and inhibit proliferation in tumor cell via genes relative to supply new experimental proofs to cancer-treating with these TCM therapies.Method:Excluding the control group ,the rest were injected single-cell suspension at the left axilla to set up SI80 model. The 120 mice were randomly divided into low-dosage group,high-dosage group,acupuncture group group,model group and control group according to their body weight. The low-dosage group and high dosage group were treated with self-made herb solution at a dosage of 3.4 ml and 1.7g/ml respectively.The moxibustion group was treated with self-made moxa stick (0.6mmX 10cm) at RN4.The acupuncture group was needled in ST36 point and round the injecting cite. These 5 groups were treated 1 time per day for 10 days successively.The model group and control group were stimulated by grasp at the same time and with the same strength as those 5 groups.At the list day. all groups were sacrificed, and the tumor tissue was taken out .Then the inhibiting-tumor rates were calculated,and the expression rates of bcl-2, bax protein and PCNA were determined by immunohistochemical technique with computerized image processing The data were analyzed with one-way ANOVA by means of SPSS 11.0.Result:1.The inhibiting-tumor rates of these 5 groups were low-dosage group 30.5%, high dosage group 49.7%, acupuncture group 37.7%, moxibustion group 34%.2. The expression rates of bcl-2 protein and PCNA in tumor cell in all TCM therapies groups was significantly lower and the expression rates of bax protein were significantly higher than those in model group(p<0.01).Conclusion:Acupuncture ,moxibustion and Chinese herbs can suppress the expression of bcl-2 and PCNA ,enhance the expression of bax and lower the ratio of bcl-2/bax in tumor cell of s180 model mice,which is one of mechanisms for these TCM therapies to exert their antitumor efficacy.