Effect Of WenDan Decoction On Lipid Metabolism In Hyperlipidemic Adult Mice And Rat

We studied the influence of WenDan Decoction on the disorder of lipid metabolism in experimental mice of acute hyperlipemia model. To prove the regulating action of WenDan Decoction for serum fat of adult mice, we studied Triglycerides(TG), Total Cholesterol (TC)> LDL-c, SOD, MDA in serum, and LA, LPL, HL in liver and fat content in faeces of adult mice, compared with Western medicine (Gemfibrozil) at the same time. Results suggest that wenDantang can markerly decrease TG, TC, LDL-c, MDA in serum of acute hyperlipermia model mice and increase the action of SOD, LPL and LA, but don't act on HL. The results also suggest that the impact of WenDan Decoction group was better than other groups(p<0.05).In the model of chronic hyperlipermia mice, We studied Triglycerides(TG), Total Cholesterol (TC) and HDL-c in serum, the result suggest that WenDan Decoction can decrease TG, TC in serum of chronic hyperlipermia model mice, but it can't influence HDL-c.On the basis of establishing hyperlipidemia rat model, Triglycerides(TG), Total Cholesterol (TC) in serum, activities of Lipase (LA), Lipoprotein Lipase (LPL), Hepatic Lipase (HL) in liver, parts of hemogram and hepatic LDLR mRNA levels were investigated after 21 days feeding of atherogenic diet. The results suggest that WenDan Decoction significantly reduced the serum TG,TC and increase the activity of LPL and LA, but nullity in HL. At the same time parts of hemogram index such as Red Blood Cell Count (RBC), Assay of Hemoglobin (HGB), Assay of Hematocritin Blood (HCT), Blood Platelet Count (BPC)> Platelet Distribution Width (PDW) and Mean Platelet Volume (MPV) suggest that WenDantang can lessen blood viscosity and have anticlotting effect. The result of RT-PCR test showed that high fat and high cholesterol feeding can significantly induce the reduction of LDLR mRNA levels, while WenDan Decoction can increase hepatic LDLR density. In sum, WenDan Decoction can prevent disorder of lipid Metabolism by regulating TC, TG, LDL-c through upregaulation of LDLR transcription level and improving ability of antioxidant.