| objective: An interleukin-1 receptor antagonist blocking the function of Interleukin-1 was used to investigate the role of Interleukin-1 and its receptor antagonist in skeletal muscle ischemia-reperfusion injury(SMRI).Methods : Twenty-four healthy male Sprague-Dawley rates, weighing 250 to 300g. were selected and randomly devided into group A, B,and C.The group A underwent anesthetization and external jugular vein cannulation alone;the Group B underwent 4 hours of left hindlimb ischemia followed by 4 hours of reperfusion;the Group C also underwent ischemia and reperfusion, and was treated intravenously with IL-1ra (2mg/kg)at the time of reperfusion.Levels of IL-1mRNA of skeletal muscle and lung were determined by reverse transcription-polymerase chain reaction.enzyme-linked immum-osorbent assay was performed for plasma level of IL-1β ,TNF- a .The levels of lactate dehydrogenase(LDH), creatine kinase(CK),malondialdehyde(MDA)and thmyeloperoxidase(MPO) activity in skeletal muscle and lung were measured by colorimetry respectively ,and the expression of intercellular adhesion molecule-1(1CAM-1) protein in endothelium was assessed by immonhistochemistry.The oedema degree was quantified by calculating the wet/dry weight ratio of skeletal muscle.Skeletal muscle and lung were also observed histologically and ultrastructurally.Ruslts: SMRI contributed to increase the level of IL-1 mRNA in skeletal muscle and lung as well as IL-1β, TNF-a in plasma significantly.The level of CK ,LDH , MDA , MPO and wet/weight ratio increase significantly and the expression of 1CAM-1 in vascular endothelium of skeletal musle and lung was also markedly upregulated by SMIR. The levels of IL-1 mRNA , IL-1β ,TNF- a , CK , LDH ,MDA, MPO and wet/dry weight ratio were remarkedly decreased after intravenous administration of IL-lra and thus attenuated the pathological injuries in skeletalmuscles and lungConclusion SMIR enhanced the synthesis of IL-1,which induced the injury ofskeletal muscle and the lung. |
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