Research On Self-Assembled BLMs And Its Application On Biosensors

The self-assembled BLMs is giving more attentions by biologists and chemists. Lipid molecules have especial structures - with a hydrophilic head and two hydrophobe tails. In the appropriate conditions, great deal of lipid molecules can assemble into BLMs. The structure and its application on biosensors were investigated. The main works and conclusions are included as follows:(1) The BLMs was studied by using electrochemistry analyzer ; A novel method has been developed for accurate measurements of membranes capacitance and resistance on the solid-supported bilayer lipid membranes by cyclic voltammetry technique. An improved equivalent circuit for the s-BLMs was proposed, which is based on the solution for the coupled equations. This approach greatly facilitates the determination of the s-BLMs electrical properties by CV. The curve fitting procedure for testing the reliability of this method has been elaborated by using a newly designed BLMs simulator. The mechanics and characteristic of formation of s-BLMs have been studied. (2) The BLMs was studied by using ellipsometry imaging method;The layer structure of lipid membranes formed on silicon chip was studied with the use of the ellipsometry imaging method. It was found that the molecular density in the outer area was higher than that of in the center area of the membranes, and some theoretical analysis was given. It seems that by using effective facilities, supported bilayer lipid membranes can become useful models for the studing of membrane biology, and the significant application in the biosensor will be studed.(3) The model used as cell sensor based on BLMs was worked ;A new model was given, which based on s-BLMs can related to the cells in the follows. So some signal can detected by such a approach. (4) The widely used experimental models of biomembranes were given. BLMs on various supports have given considerable importance in the development of biosensors and molecular devices. These supported membranes have been deposited on a number of substrates such as gels and stainless steel. This part summarizes the past research and describes the current work on s-BLMs as potential biosensors and molecular devices.