The Effect Of Substance P Induced Histamine Release In Human Hypertrophic Scar's Mast Cell

Scar is a normal prosthetic process of tissue damage. But hypertrophic scar not only can destroy patient's appearance, result in malfunction but also can induce pain, pruritus and paresthesia. Though the pathogenesis of hypertrophic scar is still unclear, more and more researchs show that substance P and histamine release of mast cell may play important roles in the development of HS,and may be some kinds of mediums. But there is no reports about the relationship between substance P and mast cell in HS till now.The objectives of this study are to detect a ration research of substance P promoting histamine release of mast cell in HS,to observe morphology of substance P nervous fiber and mast cell. then to discuss the effect conditions of substance P nervous fiber to mast cell in HS, the pathogenesis of hypertrophic scar's formation and pruritus what substance P nervous fiber and mast cell make.To expand a new prevention and cure domain for HS.In this case Our study use various concentrations of substance P or various concentrations of calcium under various affect time to assay histamine release of mast cell using immunofluorescence.Observe shape and relationship of substance P nervous fiber and mast cell in HS after immunohistochemistry and toluidine blue,and find out ultrastructural change of mast cell in Hs under electron microscope .The main results and conclusions are as fllows:1. Substance P induced histamine release of mast cell in a dose-dependency and time-dependency. Nearly 90% of histamine release occurred within 15 min. Substance P promoting histamine release of mast cell in HS strongs than in normal skin.2. The dose of calcium can affect substance P promoting histamine release of mast cell.3. Substance P and mast cell have close relationship in HS,as 'pearls' appearance, significant than normal skin.4. Compare with normal skin,amount,shape and relationship of substance P and mast cell in HS has significant difference,indicate that substance P and mast cell may play an important role in the development and pruritus of HS.