The Effects XGD-1 On The Proliferation Of Human Peripheral Blood T Lymphocyte And The Expression Of IL-2R

Objective: To explore the immunosuppressive effect of XGD-1 and its mechanism. Methods: Different concentrations of XGD-1 were added to PHA or ConA induced human peripheral blood T lymphocyte . 72h later modified MTT assay was employed to test the effect of XGD-1 on T cell proliferation . Flowcytometry was used to examine the effect of XGD-1 on the expression of IL-2 receptor on the surface of T cells individually at 48h and 72h . And the effects of XGD-1 combined with cyclosporine A(CsA) on the proliferation of T-lymphocytes and the expression of IL-2R were also investigated. Results: In the concentration range of 0.2-25μg.ml-1, XGD-1 exerted marked inhibitory effect on PHA or ConA induced T cell proliferation, which was proportional to dose. Flowcytometry showed that XGD-1 inhibited the expression of IL-2R . And the percentage of IL-2R Positive cells after stimulation of PHA decreased from 47.67% to 25.03% in the presence of XGD-1(1μg.ml-1) . XGD-1 and CsA had synergism in inhibition of T cell proliferation and IL-2R expression . Conclusions: The study suggested that XGD-1 hadimmunosuppressive effect . The suppressive effect of XGD-1 on T cell proliferation was most probably mediated by decreasing IL-2R expression.