Expression Of Fas And MDR1 Related To The Response Of Chemotherapy And Prognosis In Cervical Carcinoma

Objective: To evaluate the relationship between the expression of Fas and MDR1 and the response to chemotherapy and prognosis in cervical carcinoma. Methods: hi this investigation,sixty-eight cases from thirty-four cervical carcinoma patients were evaluated before and after chemotherapy respectively. The expression of Fas protein and MDR1 mRNA were detected by the methods of immunohistochemistry and in situ hybridization. Results: The positive expression rates of Fas protein in cervical carcinoma in the pre-chemotherapy and post-chemotherapy groups were 85.3% and 100% (P<0.05). The positive expression rates of MDR1 mRNA in the same groups were 35.3% and 79.4% (P<0.01). There was significant difference between the two groups. The positive expression rates of MDR1 mRNA hi the chemotherapy-sensitive and non-sensitive groups were 60% and 94.7%. There was significant difference between the two groups(P<0.05). The positive expression rates of MDR1 mRNA in high- differentiation, middle -differentiation and low-differentiation groups were 40% , 78.9% and 100%. There was significant difference among the three groups(P<0.05). Conclusion: The expression of Fas protein in the post-chemotherapy cervical carcinoma was significantly stronger than that of pre-chemotherapy. This indicates that chemotherapy could induce high expression of Fas protein in cervical carcinoma.The expression of MDRlmRNA in the post-chemotherapy cervical carcinoma was the same as the Fas expression. This suggests that chemotherapy could induce over-expression of the MDRl gene to increase drug resistance of tumor cells.The expression of MDRlmRNA provided a negatively-correlated index of chemotherapy.Lower cell differentiation correlates with higher positive expression of MDRlmRNA. This suggests that the expression of MDRl gene is related to prognosis and chemotherapeutic sensitivity of cervical carcinoma.