Establishment Vaginal Dysbacteriosis In Mice Model And Adjustment With Lactobacilli

Objectives: Bacterial Vaginosis (BV) is considered the most common vaginalinfection in women, the incidence of BV is 15~50%: Microbiology showed that BV isa clinical syndrome caused by replacement of the normal lactobacilli producinghydrogen peroxide in the vagina with high concentrations of characteristic sets ofaerobic and anaerobic bacteria, for instance Gardnerella vaginalis, Streptococcus,Bacteroides and Escherichia coli etc. Number of organism in the healthy vaginalindigenous flora is 105~106/ml in vaginal flush, but 109~1011/ml in vaginal flush withBV. Clinical situation of BV was absence of vaginal mucous membrane inflammatorysyndrome. Published studies indicate that the drug of choice for therapy is an antibiotic.Although the therapy of an antibiotic relieved symptom of BV temporarily, fartherkilled few Lactobacilli. Imbalance of vaginal micro-ecosystem became very severe, sothat BV repeatedly recurred after the therapy. Lactobacilli are major constituent of thenormal vaginal flora and are believed to play a protective role in guarding against thevaginal infection to the pathogens and adjustment of vaginal dysbacteriosis, whichproduced a marked effect through combination of competitive exclusion and inhibiterproduction. Recovering the balance of vaginal micro-ecosystem and normally vaginalenvironment were the key point of curing BV.Establishment of vaginal dysbacteriosis in the animal model was the base of exploring pathogenesisx clinical therapy and new physic development of BV. At present domestic report is a vacuity, foreign related report little. First the purpose was to establish a mouse model of vaginal dysbacteriosis. Establishment of the animal model had an important significance to offer experimental evidence of the further investigation. Second purpose was that observed adjustment of vaginal dysbacteriosis of model mice by intravaginal inoculation of Lactobacilli debrudckii and safety of Lactobacilli debrudckii in vaginal application of healthy mice.Methods:1.Establishment vaginal dysbacteriosis in a mouse model. Groupl was administered by intravaginal amoxicillin solution and E.coli suspension in the mice; group2 was administered by intravaginal E.coli suspension in the mice. We evaluated reliability and stability between two groups by dynamic flora analysis of vaginal colonization with E.coli in mice.2. Adjustment of Lactobacilli debmdckii to the vaginal dysbacteriosis in model mice. When a persistent colonization with E.coli was established, attempts to decolonize were made in the treated group with suspensions of Lactobacilli. We evaluated adjustment of vaginal dysbacteriosis by Lactobacilli intravaginal inoculation of mice by bacterial flora analysis and pathologic findings of the vaginal flush of mice. The experiment was an application of vaginal dysbacteriosis in a mouse model.3. Safety of Lactobacilli debrudckii vaginal application in mice. Mice were inoculated intravaginally with quantitative (106CFU/ml, 108CFU/ml and 109CFU/ml) Lactobacilli debrudckii. We observed and compared the relations among four groups.Result:1.Establishment vaginal dysbacteriosis in a mouse model. The results showed that intravaginal administration of amoxicillin-induced E.coli produced a longer duration of E.coli colonization in mice vaginal flora and a higher level. Number of E.coli in group 1 was significantly increasing of the vaginal flora compared with untreated amoxicillin-induced administrated group2 (P<0.05). The persistence of amoxicillin-induced E.coli colonization was monitored for 7 days. There were only small day-to-day variations, usually within 1 log, however group2 over 3 logs. The pathologic findings: a variety of severe inflammation of mice vagina in groupl. We may think that the method of the established vaginal dysbacteriosis in groupl was successful and reversible.2. Adjustment of Lactobacilli debrudckii to the vaginal dysbacteriosis in model mice. Treated group with suspensions of lactobacilli was significantl...