A Study On Expression Of KAI1,E-cadherin And Survivin In Colorectal Carcinomas And Its Prognostic Significance

Objective: To explore the roles of KAI1 , E-cadherin(E-cad) and survivin in the development and prognosis of colorectal carcinomas (CRCs) and the relationship between KAI1 and E-cad by observing the characteristics of the expression of KAI1 , E-cadherin and survivin in CRC.Methods: RT-PCR was used to detect mRNA expression of KAI1 and survivin of normal mucosa, transitional mucosa of colon and CRC; High sensitive Envision?and SP immunohistochemical method were used to detect the protein expression of KAI1, E-cadherin , survivin and VEGF in CRC; TUNEL method was used to test AI.Results: 1.The KAI1 mRNA levels of normal mucosa,transitional mucosa and CRC increased in turn. The KAI1 mRNA of normal mucosa was significantly lower than that of tumor.2. The protein expression of KAI1 and E-cad increased in the cases of high differentiation and living over 5 years, reduced in the cases of high TNM stage , with lymph node metastasis and distant metastasis.3. The protein expression of KAI1 and E-cad in primary CRC were coincident and significantly correlated.4. The survivin mRNA levels of normal mucosa, transitional mucosa and CRC increased in turn .The survivin mRNA of normal mucosa was significantly lower than that of tumor .The protein expression of survivin significantly increased in the cases with distant metastasis. The 5-year survival rates of patiens were lower in the cases with high survivin protein expressionthan those with weak expression; The protein expression of survivin and VEGF was coincident and significantly correlated in primary CRC;5.AI of CRC was positively associated with the 5-year survival rates of patiens. The protein expression of survivin was negatively associated with AI.Conclusion: l.The increased expression of KAIl in adenocarcinoma probably participate in carcinogenesis of CRC.2. KAI1, E-cad may possess important roles in development of CRC because of inhibiting lymph node metastasis and distant metastasis and probably be a valuable marker to predict the prognosis of CRC.3. KAIl and E-cad probably coexist in a protein complex -KAI1/ E-cad complex, or there's a positive feedback between them. KAIl and E-cad gene can fiercely inhibit the metastasis of CRC in vivo. The metastatic CRC cells can express E-cad again but not KAI1.4. The increased expression of survivin in adenocarcinoma probably participate in carcinogenesis of CRC. Survivin possibly correlated with hematogenous metastasis of CRC. There may be a positive feedback between survivin and VEGF. Survivin may be valuable reference to predict the prognosis of CRC.5.Inhibition of apoptosis is a important factor to decide the prognosis of CRC. Survivin can significantly inhibit the apoptosis of CRC. Survivin facilitate the development of CRC and decide its prognosis may be through inhibiting apoptosis.