| Acetylcholine has been regarded as a kind of wildspread neuronal transmitter. It has many important functions, it mainly puts excitatory effect on the central neurol system. Some evidence show that epileptic patient has higher extracellular acetylchoine concentration in brain than the control people. And seizures can be induced by activation and suppressed by the inhibition of acetylcholine receptors. The change of extracellular acetylcholine concentration and receptor structure is epileptogenic. The aim of this experiment is to evaluate acetylcholine receptor antagonist, scopolamine' s anticonvulsant and neuronal protective effects. To detect the concentration of excitatory and inhibitory amino acid transmitters in brain in rats to analysis its anticonvulsant mechanisms.This experiment is devided into two parts. In the first part, we use direct cortical stimulation animal model, to observe the influence of scopolamine on the seizure threshold and neuronal protective effects in hippocampus. In the second part, chemical3kindling animal model is used to detect the effect of scopolamine on seizure activity and the concentration of excitotary and inhibitory amino acid transmitters in brain. The results: the threshold increased after injection of scopolamine and after-discharge seizure decreased. But the anticonvulsant effects of scopolamine is lower than Carbamazepine. To the pathological slices, the neuronal injury is much better in scopolamine group than that group which don' t accept any antiepileptic medicine. Scopolamine decreased seizure behavior of kindled model. The concentration of excitatory neuronal transmitter is decreased and the inhibitory neuronal transmitter is increased in scopolamine group, compared with the group which accept only picrotoxin. The conclusion: scopolamine has anticonvulsant effect, it could be used in the clinical treatment to the epileptic patient.
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