Analgesic Component Of Toxin Of Agkistrodon Halys Pallasanalgesic Function Research And Its Mechanism Elementary Explore

Objective To purify Analgesic component of Toxin of Agkistrodon halys pallas and observe its toxicity; at the same time, validate ease pain of nalgesic component .The approach of discussion its effect by nerve centre or outer circumference .Method (1) Jiangzhe snake is difficult in purification by singularity coluniation. So we make use of its otherness of electric charge nature and molecular weight ,adopt three step asunder method, namely original asunder using hydronium exchange earlier, then using two type molecule filtrate and asunder .at last, we ascertain ease pain component making use of little mouse .(2)To appraise the securiy by the experiment of its urgent toxicity , body depending and its enduring.(3)Vary dosage component inject by muscle, and set up negative nature comparison group, masculine comparison group ,at the base of ease pain experiment method,evaluate its effect to heat stimulate , chemistry stimulate and mechanism stimulate.(4)To judge its action positon in nerve centre or outer circumference by the mean of rat side ventricle injection rat foot stable press stimulate , rat spinal cord destroying, and at the same time, wemensurate the change of the blood serum morphia after injectingdrug.Result (1)Finally, apart singularity chromatogram apex, and its pure degree is 97.5%.the separating effect of the molecule griddle lie on its discrepancy in molecular weight and the velocity of flow of the fluid mostly. The result indicate that the velocity of 0.5ml/min is appropriate for separate columnia-tion, using three separate method can apart and purification for this component .after cation exchange and size exclusion, we got NO 4 .It has stronger analgesic function and it was called as C4(Peak NO 4 after cation exchange column separation). The molecular weight of C4 protein was 16.6 KD, isoecectric point was 8.8.It is basic proteins.(2)LDso of Analgesic component was 13.6mg/kg by muscle injection. Equal to 35 times of clinic apply clew to C4 Analgesic component is safety and effective, through test, we testify that Analgesic component has no kickback like morphia after continuum 7 days injection.(3) Analgesic C4 component of Toxin of Agkistrodon halys pallas can control all kind of pains such as heat stimulate - chemistry stimulate and mechanism stimulate. The pain limit increase rate of three group of C4 Analgesic component all excellent to the group of NS. The difference is prominence(p<0.05).at the same time,the analgesic function of C4 Analgesic component reaching best appeared after 60min injection, it is function later butperdure to sutra comparison.(4)Through experiment, we prove C4 Analgesic component is independent of acetylcholine, reser-pine can increase pain limt rate, Naloxone can partially reduced the effect of C4 Analgesic component, and C4 Analgesic component act through adjust nerve centre, it showed striatum, thala-mus, red nucleus might be organs of C4 Analgesic component central action.Conclusion The method of purifying C4 Analgesic component was definitude ,and it was easy repeat ;it was a kind of lower toxic and no body dependence and tolerance after long use. it is effective drug and it act by adjust nerve centre, C4 Analgesic component is more deserved empolder and research .