Study On Gluten Influence Diabetes Incidence And Enteric Bacterial Profile In Non-Obesity Diabetes Mice

Diabetes is a integrated disease caused by many interacted factors which lead to the turbulence of the glucose, amino acid and the lipid metabolism, due to the lack of insulin hyposecretion or insulin cells metabolism limitation. Its basic characteristic is the abnormal rise of the glucose content in the blood circulation. The further development of diabetes will cause all kinds of serious or chronic complication, which will threaten the healthy body and even endanger the life. Diabetes is one of the chronic diseases which are very harmful to mankind and animals. It is hard to cure in clinical, and the rate to get such a disease is increasing day by day. Its main reason lies in inherited factors, environmental factors and the interaction between the two factors. The causes and pathogenesis of diabetes are very complicated, which is still unknown up till now. The exploration in pathogenesis of diabetes is still the hotspot to study.The experiment adopted the non-obesity diabetes mice as the model. They were fed the standard diet with gluten as well as the gluten-free diet, and observed whether the gluten could influence the onset and incidence of diabetes. We cultivated the appendices matter from caecum of the diabetes mice and healthy ones with selection or non-selection media, analyzed the bacterial species and the quantity of the dominant bacteria existing in the intestinal flock. The result of the experiment showed as follow:1. The gluten was the factor inducing diabetes. The peril suffering from diabetes is nine times higher in the mice feeding of gluten than that in the mice feeding of gluten-free.2. Whether the diet contained gluten or free had little effect on the change of the mice. 3. The analysis of appendices matter from caecum showed that there was no distinctive change in species of the dominant bacteria as far as the intestinal bacterial profile of the diabetes mice and normal ones is concerned. 4. The gluten fed mice had significantly more aerobically and microaerophilically cultivated bacteria in the intestine than non-gluten fed mice. The diabetic mice had significantly more microaerophilically and anaerobically cultivated bacteria in the intestines than non-diabetic mice . No differences in the numbers of Enterobacteriaceae were found.5. The gluten-fed mice had significantly more Gram-positive bacteria in the intestine than non-gluten fed mice...