The Effect Of Radiosensitization Induced By Bufalin On CNE-1

ObjectiveChemical agents have not only anti-cancer effects, but some of them have radiosensitive enhancement effects. Bufalin is a new Chinese traditional medicine which can induce differentiation and apoptosis and inhibite growth of cancer cells. It has been proved that bufalin has anti-cancer effects on human melanoma, head and neck squamous cancer, gastric cancer, colon cancer, prostate cancer and leukemia cells. At present research about radiosensitization of bufalin has not been reported. The purpose of this experiment was to observe the radiosensitive effect of bufalin on CNE-1 nasopharyngeaJ cell line.MethodsAfter obtaining ID50 of bufalin on CNE-1 through MTT, we exposed CNE-1 to bufalin in two concentrations for 24 hours which were assigned to 6 groups. In the irradiated groups, 2Gy, 4Gy, 6Gy and 8Gy X rays, 6MV. were given with single dose by accelerator. The cell survival curves and SERs (sensitive enhancement ratio) were obtained with clonogenic assay, after analysis with SPSS software.Results In pure medicine group, survival fraction of CNE-1 decreased asconcentration of bufalin increased, and its ID50 is 2.5 10-7M. Bufalin of 2.5 10-9M and 2.5 10-7M both had radiosensitive effects on CNE-1 exposed for 24 hours. SERs of which are1 .41 and 1.42.ConclusionBufalin of 2.5 10-9M and 2.5 10-7M both had radiosensitive effects on CNE-1 exposed for 24 hours, and the effects increased with increasing concentration.