The Study Of The Relationship Between GLU298ASP (894Gâ†'T) Single Nucleotide Polymorphisims At Exon 7 Of The Human Endothelial Cell Nitric Oxide Synthase (heNOS) Gene And Diabetic Nephropathy

Objective To investigate the relationship between the GLU298ASP (894G-*T) single nucleotide polymorphism at exon 7 of the human endothelial cell nitric oxide synthase (heNOS) gene and diabetic nephropathy in Northern HAN patients with type 2 diabetes mellitus.Methods The GLU298ASP (894G^T) allele and genotype at exon 7 of the human endothelial cell nitric oxide synthase (heNOS) gene in 238 subjects were examined by polymerase chain reaction ,agarose gel electrophoresis and restriction enzyme BanII, of which there were 138 cases of type 2 diabetes mellitus and 100 normal subjects. The group of type 2 diabetic patients was devided into two subgroups:diabetic nephropathy group (DN+ group, 61 cases) and diabetic non-nephropathy group (DN- group, 77 cases) according to urinary albumin execretion rate (UAER). The allele and genotype among each group were compared with higher concentration agarose gel electrophoresis. According to the clinical biochemical results of diabetic patients, the relatioship between the clinical biochemical results, allele , genotype and DN was investigated by logistic analysis.Results The frequencies of both allele T and genetype TG were significantly higher in DN+ group than those in DN- group and normal subjects (P<0. 05) , but there was no significant difference between DN- and normal subjects (P>0. 05 ). Statistics analysis showed that allele T and genotype TG were associated with diabetic nephropathy in type 2 diabetic patients(P=0. 022 and 0.020 ,respectively).And Logistic regression analysis suggested that the GLU298ASP (894G-T) single nucleotide polymorphisms at exon 7 of heNOS gene may be an independent risk factor for diabetic nephropathy(OR=2. 919).Conclusion This findings suggested that the TG genotype of GLU298ASP (894G -T) single nucleotide polymorphism of heNOS gene may be associated with diabetic nephropathy in Nortern HAN patients with type 2 diabetes mellitus . It may be an independent risk factor for diabetic nephropathy and can be served as an inherited marker of susceptibility of DN.