The Changes Of Gastric Mucosa Acetylcholinesterase And Serum Nitric Oxide Level And Their Significance In Patients With Diabetic Gastropathy

Objective The movement of gastrointestinal tract is controlled by the cholinergic and nitrergic inneration in enteric nervous system. This study was done to examine the changes of gastric mucosa acetylcholinesterase(AchE) and serum nitric oxide(NO) level in patients with diabetic gastropathy to investigate their effects on diabetic gastropathy. Methods Seventeen diabetic gastropathy patients and the 14 sex- and age-matched normal controls were included in this study. Diabetic gastropathy patient's symptom scores were assessed; the serum NO concentration was determined in all subjects by the immunocolorimetry, using nitrate reductase to reduce nitrate as nitrite; the correlation was analyzed between diabetic gastropathy patient's symptom scores and serum NO concentration. After gastroscopy and biopsy in gastric mucosa were performed in all subjects, the distribution of AchE in the gastric mucosa was determined by the histochemistry method and observed by the Olympus-BX50 color image spectrograph. Results AchE positive color was brown. Area of AchE positive staining cells which were mainly located in the gastric mucosa of the patients with diabetic gastropathy were markedly lower than that of the normal controls(974.10 56.19 micro.m2,1057.94 87.68 micro.m2), P<0.005.Serum concentration of NO was also significantlydecreased in the patients with diabetic gastropathy than in controls (59.82 9.62 micromol.L-1,79.48 11.30 micromol.L-1, P<0.001; there was a significantly inverse correlation between symptom scores and serum NO concentration in patients(r =-0.893,P<0.01). Conclusions The results of this study indicated that patients with diabetic gastropathy might be associated with the level of NO in the serum and AchE in the gastric mucosa, low serum No level might lead to diabetic gastropathy symptoms.