| Objective To explore a relatively simple and effective method for studying ischemia-reperfusion(I/R) injury of the intrahepatic bile duct (IBD) and investigate the protective effect of radix salviae miltiorrhizae (RSM) according to the injury mechanism, which provide scientific theories and methods for preventing and curing complications of the IBD after liver operation, especially liver transplantation. Methods Based on the model of partly rat-hepatic (70%) I/R injury in vivo, the common bile duct was cannulated with a fine catheter for bile collection. RSM was abdominally injected in one experimental group instead of saline in corresponding control group before I/R. Glucose(Glu) content and gamma-glutamyltransferase (GGT)activity in bile of each rat before and after I/R were assayed . GGT in serum of two groups were tested as well. Pathologicalchanges of the IBD cells were observed through light microscope and electronmicroscope. Results A marked ultrastructural alteration of IBD epithelia was found indicating the lesion induced by I/R. Glu content and GGT activity significantly increased after I/R (P<0. 05), while RSM lowered the rising levels of them (P< 0. 05) by alleviating billiary epithelium damage. Conclusions The model and method may be useful to study IBD injury; Such indexes as Glu, GGT and so on can be applied to examining the I/R injury of IBD; RSM has an effect on protecting biliary epithelia against I/R injury. |
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