Association Between Polymorphism In Gene For Microsomal Epoxide Hydrolase And Susceptibility To The Chronic Obstructive Pulmonary Disease

Objective We investigated whether polymorphism in gene for microsomal epoxide hydrolase (mEH) had any bearing on individual susceptibility to the development of chronic obstructive pulmonary disease in Han nationality of north China. Method The genotypes of 55patients with COPD and 52 healthy smoking control subjects were test with polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (PCR-RFLP) for tire mEH gene.The frequencies of polymorphic genotypes of mEH were compared in patients with COPD and healthy smokers. Result (1) .In COPD group mEH exon 3 homozygous wild-type,heterozygote and homozygotemutant was 27.3%,27.3% and 45.5% respectively and exon 4 homozygous wild-type,heterozygote and homozygote mutant was 72.7%, 18.2% and 9.1% respectively. The frequencies of polymorphic genotypes in exon 3 and exon 4 of mEH showed no differences between the COPD group and the control group (P>0.05) . (2) The frequency of allele(113His) in exon 3 of mEH was significantly higher in severe group than medium and light group in COPD (P<0.01) . Conclusion (l)Genetic polymorphisms in mEPHX is not associated with development of COPD in Han nationality of north China.(2) Our study reveas suggest that allele frequency(113His) in exon 3 of mEH locus is in association with COPD severity.