The Changes Of Nitric Oxide Synthase Positive-Neurons In Hippocampal Formation Of Female Rat After Ovariectomy

The changes of Nitric Oxide Synthase Positive-Neurons in Hippocampal Formation of female Rat After Ovariectomy.In the present study, we adopted the SABC immunohistochemical technique to study the changes of morphology and distribution of nitric oxide synthase positive neurons of hippocampus , which were analyzed with computer graphic analysis system. The study results show: 1)there are comprehensive NOS-positive staining cell groups in all regions of hippocampal formation , pyramidal cell and granule cell are the two main cell in both reference and test groups, but in CAI and DG region small cell is more, whereas cell dendrite is shorter in test group than in reference group.2)The distribution of the neuron containing NOS in the hippocampal formation show a obvious regional difference in the reference group and text group After ovariectomy ,the number of positive neurons in CAI ,CA3 ,CA4 ,S and DG is decreased(P<0.05) ,while the number of positive neurons in CA2 region has no remarkable changes(P>0.05) .So it suggests that estrogen can stimulate the compose of nitric oxide ,and estrogen may influence the learning and memory by affecting the expression of NOS in hippocampus.