The Effect Of Portal Vein Chemotherapy On Circulating Blood Micro-metastases Of Colorectal Cancer Patients

objective To evaluate the effect of the short- term portal vein chemotherapy on inhibiting the circulating blood micrometastases of colorectal cancer patients.Methods Portal vein blood and peripheral blood of 20 colorectal cancer patients were drawn after operation and after postoperative seven-day portal vein chemotherapy, respectively. Nested RT-PCR was used to detect CEAmRNA in these blood samples. Besides,20 colorectal cancer patients who were only done operation were choosen randomizedly as the control group.The CEAmRNA expression in 20 healthy persons were as negative controls and that in VOLO cancer cells plant as positive controls. Results There was a significant difference of CEAmRNA positive expression in blood between patients with liver metastasis and non-liver metastasis.However,there was no significant correlation among other clinicopathalogic characteristics.20 patients in perfusion group completed chemotherapy.3 patients had transient gastrointestinal upset. Before chemotherapy,CEA-mRNA positive rate in the perfusion group was 60%(12/20) in portal vein blood and 50%(10/20) in peripheral blood.After chemotherapy 6 (Dukes' B stage 2 and Dukes'C stage 4)of 12 portal vein blood positive patients and 6 (Dukes B stage 2 and Dukes C stage 4)of 10 peripheral blood patients turned to negative.There was a significant difference of CEAmRNA positive expression in blood before and after chemotherapy.(p<0.05) Howover,in control group,the statistic result was no significant difference.CEAmRNA expression in all heathy persons were negative,while in VOLO cancer cells plant,all positive. Conclution Expression of CEAmRNA can indicate the spread of tumor cells in patients with colorectal cancer. postoperative portal vein chemotherapy was safe and reliable and could inhibit liver metastases of colorectal cancer patients,specially for Dukes B and C stage patients. The detection of CEAmRNA expression associated Dukes staging is useful to determine the necessity of postoperative portal vein chemotherapy.