The Quantitative Change Of NOS In Overtraininged Rat's Hippocampus CAI And Hypophysio-Trophic Area

The pathological mechanism of nerve-spiritual symptom and nerve-endocrine symptom in overtraining syndrome (OTS) is the hot research in the world now. The study is about the changes of nitric oxide synthase (NOS) in central nervous system's (CNS) hippocampus CA1 arid hypophsio-trophic area (HTA) after overtraining, and it is also about the relation between the changes and nerve-spiritual symptom and nerve-endocrine symptom in overtraining syndrome. However, few researches about above-mentioned subjects have been carried out up to now. The experimental research studies the following problems over the male rats after overtraing by establishing the model of the male rats: (1) The change of the quantity of NOS immunoreactive nerve cells and the activity of NOS in hippocampus CA1; (2) The change of the quantity of NOS immunoreactive nerve cells in some nucleus of HTA's, including ventromedial nucleus of the hypothalamus (VMM), arcuate nucleus of the hypothalamus (ARE) and medium eminence (ME). The experiment aims to discuss the changes of NOS in CA1 and HTA after overtraining, and makes a further discussion of the pathological mechanism of nerve-spiritual symptom and nerve-endocrine symptom after overtraining. The experiment uses the general male Wistar rats from the Animal Center of Shanxi Medical University, and the rats are divided into experimental group and contrastive group at random. The male rats of the experimental group have been trained for seven weeks. We trained them to swim with load in order to establish the overtraining syndrome animal model. At the same time we take the samples from the experimental group and the contrastive group after the animal model had been established. The method of taking samples is: (1) Peeling the brains from the rats, and making them into slices. The thick of every slice is 40 micron. Then we make the slices of CA1, VMH, ARH, and ME, employing the method of immunohistochemistry. Then we compare the nucleus of the experimental group and the contrastive group in immunoreactive nerve cells' shape and quantity. The method of obtaining quantity: selecting the same level slices of every rat, and choosing three 鈥?eye shots at random, then counting the number of cells. (2) The number of NOS immunoreactive nerve cells in overtraining male rats' CA1 is more than those of contrastive group rats (P<0.01), and we find that NOS immunoreactive nerve cells in overtraining male rats' CA1 are bigger than those of contrastive group rats. The cells are middle neurons. The NOS activity in CA1 of overtraining rats is higher than that of contrastive group rats (P<0.01). (3) NOS immunoreactive nerve cells cannot be found in ARH and ME of the two groups.The results of the experiment prove that (1) The increase in NOS might well be the character of nerve-spiritual symptom and nerve-endocrine symptom while overtraining syndrome happens; (2) NOS maybe participate in the formation ofovertraining syndrome in CA1 and HTA. (3) NO maybe participate in and induce the functional turbulence of hypothalamus and neuron tumefaction in hippocampus, then leads to the damnification of hippocampus, even it maybe participate in the vicious circle of hippocampus' damnification and high glucocorticoid (GC). So it is very necessary to study the subject of NOS' participating in overtraining syndrome, and the study can provide guidance for defending and curing nerve-spiritual symptom and nerve-incretionary symptom in overtraining syndrome. The experimental report is not found in our nation.