| OBJECTIVE: To study the effects of peripheral nerve allograft with different time subcutaneous preservation on sciatic nerve regeneration in rats.METHODS: 55 wistar rats were used in this experiment which were divided randomly into 5 group: the experimental group( group A, B, C, n=10 ), the control group (group D, n=10) and the donor group (group E, n=15). In the experimental group, a 15mm segment of sciatic nerve harvested from donors was separately inserted into subcutaneous compartment on the left thigh . After 1 week's (Group A), 2 weeks' (Group B) and 3 weeks' (Group C) preservation, the segment of sciatic nerve in subcutaneous compartment was removed and was transplanted into a 10mm defect of right, which was made immediately. In the control group, a 10 mm sciatic nerve defect was made and immediately repaired in situ on the right thigh. The function of the sciatic nerve was evaluated by SFI(sciatic functional index) in 2 ,4,6, 8,10 and 12 weeks after operation. The histological and electrophysiological examination were done in 12 weeks after operation.RESULT: After the operation, SFI descended gradually. After 12 weeks of operation, the SFI of group A and group D was minimum and had significant difference compared with group B and group C (p<0.05).There is no significant difference between group A and group D. A large number of myelinated nerve fibers and a small number of unmyelinated nerve fibers regenerated in group A and group D. The number and the structure of regenerated nerve were similar to the original. The number and size of regenerated axon had significant difference with group B and group C (p<0.05). There is no significant difference between group A and group D. The conduction velocity and the latent period of motor nerve were significant difference between group A,D and group B,C (p<0.05). There is no significant difference between group A and group D. CONCLUSION: The nerve allograft with 1 week's subcutaneous preservation can promote nerve better regeneration. |
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