| Malignant transformation of cell and occurrence and development of tumor correlate with the extracellular matrix(ECM). Fibroectin(Fn) is large, multi-functional glycoprotein and is the important component of the extracellular matrix (ECM). It is widely distributed on the surface of cell in tissue fluids in connective tissue and in basement membranes. FN can be divided into two major forms: Plasma ( pFn) and cellular fibroectin ( cFN). cFN differs from pFn in having the so-called extra domain sequences A or B in the molecule. Plasma FN is produced by hepatoeytes, while cFN is produced locally. It plays a major role in various fundamental biological processes such as tissue organization, cell adhesion and migration, cell differentiation, maintenance of normal cell morphology, cytoskeletal organization, embryogenesis, hemostasis and thrombosis, and wound - healing. Recently many studies focused on fibroectin have suggested that fibroectin correlates with tumor occurrence and development.The mortality of gastric cancer is highest of malignant tumors in our country, so early diagnosis and early detection have great significance in prevention and treatment of gastric cancer. It is important to find valid screening methods with the purpose of decreasing mortality of gastric cancer. The aim of this study is for the first time and large samples to investigate the clinical significance of serum cellular fibronectin concentrations in gastric carcinoma and precancr gastric diseases and provide theoretical index for screening and early diagnosis of gastric cancer. The infection of Helicobacter pylori is the direct cause of chronic activating gastritis and there is intimate relationship between H. pylori infection and gastric cancer and precursors of gastric cancer. So H. pylori has been named as first class carcinogen. It was unclear how can H. pylori play roles in occur-rence and development of gastric cancer. It is unknown whether the serum cellular fibronectin concentration is related with H. pylori associated gastric cancer.In this study, we investigated the relationship between the serum cellular fibronectin concentrations and H. pylori associated gastric diseases. So the aim of this study is to:1. To investigate the serum cellular fibronectin concentrations of different gastric diseases.2. To investigate the relationship between the serum cellular fibronectin concentrations and H. pylori infection.Materials and Methods1116 serum samples were examined, which came from gastric screening patients of the Zhuanghe region, Iiaoning province with high incidence of gastric cancer and patients of gastroscopy and operation in the First Affiliated Hospital of China Medical University. There were 612 cases of male, 504 cases of female, average age 50.71 years. Blood was collected by venepuncture and samples were stored at - 80C. The concentrations of serum cellular fibronectin and serum IgG antibodies to H. pylori were measured by enzyme - linked enzyme -linked immunosorbent assay (ELISA). All gastric mucosal biopsies were identified for pathology diagnosis by HE stain. The cases included 56 cases of normal subjects, 339 cases of superficial gastritis, 412 cases of gastric ulcer or erosion, 144 cases atrophic gastritis and 165 cases of gastric cancer.Results1. The median concentration of the serum cellular fibronectin of normal subjects was 0. 52 mg/l and the median concentration of gastric cancer was 1. 20mg/l. The serum cellular fibronectin concentration increased gradually from superficial gastritis, superficial gastritis, gastric ulcer or erosion, atrophic gastritis to gastric cancer. The serum cellular fibronectin concentration of gastric cancer was significantly high than normal subjects and gastric benign diseases (P< 0.05). The serum cellular fibronectin concentration of gastric benign diseases is distinctly high than normal subjects ( P < 0. 05 ). There was not statistically significant among the gastric benign diseases( P >0.05 ).2. To investigate from degree of differentiation and... |
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