Study On Maternal Blood,Placenta And Cord Blood Lipoprotein (a) Levels In Patients With Pre-eclampsia

PrefacePre - eclampsia is a kind of disease peculiar in gestational period, which severely does harm to both mother and infant. When the clinical manifestation of pre - eclampsia is distinct, it has always been followed by acute atherosclerosis (AS) in spiral arterioles and the extensive damage of vascular endothelia. Lipo-protein(a) [ Lp( a) ] is closely related to the damage of vascular endothelial cells and AS. So far, people have only studied the levels of Lp(a) in maternal blood, and there is no report on Lp(a) levels in placenta and cord blood. This study is a quantitative determination on Lp(a) levels in maternal blood placenta and cord blood of patients with pre - eclampsia, though which we due to investigate their effects on pathogenesis and development of pre - eclampsia. By the way, we can approach to the interaction of them three.Patients and MethodsPatientsPatients with pre - eclampsia performed cesarean section in the third trimester of pregnancy were chosen as experimental group ( n = 31) , who were previously healthy uniparous monocyesis not parturient and without internal disease and familial hypertension history. The diagnostic criteria of pre - eclampsia were complied with the sixth edition<> edited by Le Jie. It included 16 severe and 15 mild pre - eclamptic patients. Simultaneously normal pregnant women were chosen as control group (n =21), who were per-formed elective cesarean section for breech presentation cephalopelvic dispro-portion and so on.MethodsCollection of samples. Blood sample (3ml) was collected from autecubital vein of patients before operation, who had fasted for 12 hours. During operation, blood sample was collected from umbilical vein immediately after fetus been taken out. Then, the above samples were centrifugated and then serum was separated from them. A piece of placental tissue, which was 20mm 20mm 20mm in size, was excised from the center of the placenta with aseptic manipulation. After that, the placental samples were irrigated with normal saline and absorbed moisture. All of the samples were stored at -30 and were assayed together.Detection of samples. The Lp(a) levels in maternal blood placenta and cord blood were quantitatively examined by immunotransmission assay (ITA) method. The protein content in placenta! tissues were measured using Coomassie brilliant blue assay method.Statistical analysis. Using t test to compare the difference between two groups. Pearson correlative coefficiency was used in rectilinear correlation analysis. The factor analysis was carried out by Stepwise regression.ResultsAll maternal blood placenta and cord blood Lp(a) levels in patients with severe pre - eclampsia ( mean SE, respectively 309. 681 225.485mg/L 2. 695 1. 000mg/g 10.575 3.296mg/L) were significantly higher than those in normal pregnant women ( respectively 37. 890 14. 789mg/L 0. 765 0. 889mg/g 6.905 2.800mg/L;P<0.001 P<0.001 P= 0.001). In patients with mild pre -eclampsia, the maternal blood and placenta Lp(a) levels (80. 893 35.095mg/L 1.544 0.787mg/g) significantly increased compared with control group (P <0.001 P =0.01), however, the difference in cord blood Lp (a) levels between the two groups was not statistically significant (P>0.10). The Lp(a) levels in maternal blood placenta and cord blood was found to have a statistically significant increase in severe preeclampsia group with respect tothe mild one.In severe preeclampsia group, it had a significant positive correlation between placental and maternal blood Lp( a) levels ( R = 0. 891, P < 0. 001). While there was no statistical significant correlation among Lp(a) levels of maternal blood placenta and cord blood in the control group.In stepwise regression analysis placenta Lp (a) was the first to enter into the analysis of variance model.DiscussionLp(a) is a circulating independent special lipoprotein particle, it is also an independent risk factor for AS and thrombosis. It's pathogenicity is believed to be mediated by a...