| ObjectiveEndometriosis ( EM) is a common gynecological disease affecting nearly 10% of reproductive - aged women. The cause of EM remains obscure. Recently, most investigators believe that angiogenesis is a crucial phenomenon in the development of endometriosis. Neovascularization is observed within and sur-rouding active endometrotic lesions by laparoscopy.Macrophage migration inhibitory factor( MIF) was originally described as a product of activated T - lymphocytes that inhibited the random migration of cultured macrophages. It is now well known for a variety of functions, including inflammatory and immune responses, cell proliferation, angiogensis, and tumor progression. Recent findings suggest that MIF may play an important role in endometriosis - associated angiogenesis . Increased concentration of MIF was found in eutopic endometrium , ectopic endometrium and PF of women with EM .To date,there was no reports regarding the presence of MIF in plasma of women with EM . The present study was undertaken to evaluate the levels of MIF in plasma and PF of women with EM by enzyme linked immune sorbent assay (ELISA) , to examine the correlation between MIF levels and the stage of the disease and the phase of menstrual cycle, and to determin the role of MIF in promoting the disease.Methods1. Subjects.Plasma and PF samples were obtained from 29 women with EM in this stud-y. The stage of EM was determined according to the revised classification of The American Fertility Society (12 with stage I and II ,17 with stage III and IV). Moreover, the menstrual cycle phase(proliferative or secretory) was determined according to the patients'cycle history. Control subjects (n = 10) were women undergoing surgery for tubal reanastomosis or patients with parovarian cyst or ovarian serous cystadenoma, who have no visible evidence of endometriosis and inflammation at laparoscopy or laparolomy. Plasma specimens (n = 10) and peritoneal fluid (n = 10) were collected from them, respectively.2. Collection and processing of samplesVenous blood was drawn form women with and without EM before undergoing surgery , then put into tube containing anticoagulin; Peritoneal fluids were obtained at the time of laproscopy or laparotomy before any surgical intervention. Immediately after collection, the samples were centrifuged at 3000 rpm for 10 min at 4C ,and the supernatants were aliquoted and sored at -86C until analyzed3. Measured procedure.The concentration of MIF in plasma and PF were measured by the Sandwich ELISA, following the established procedure of test kit.4. Stastical analysisUnpaired t - test was used for the comparision of means , and Pearson ?test for correlation analysis.Results1. Concentration of MIF in plasmaMIF Concentrations of plasma were markedly higher in EM patients as compared with controls ( P < 0. 01). The significant increase was seen either in EM stages I - n ( P < 0. 01) or M - IV ( P < 0. 05 ). No significantly differences were shown between the case in stages I - II and stages III-IV(p>0.05).2. Concentration of MIF in peritoneal fluidThe mean Concentration of MIF in PF was significantly higher in EM patients as compared with controls ( P <0. 01). The most significant increase wasseen in EM stages I - II. In stages III - IV, MIF concen trations slightly decreased as compared with the case in stages I -II(P<0. 01), but remained significant higer than those in the control group ( P <0. 01).3. The comparison and correlation between MIF level of plasma and that of PFSignificant differences ( P < 0. 01) and posiitive correlation ( r = 0. 823, P <0. 01) were shown between MIF level in plasma and PF of women with EM.4. Correlation between MIF levels and the phase of the menstrual cycle. The increased concentration of MIF in PF ( P < 0. 01) and plasma ( P <0. 01 ) of women with EM was markedly more significant in the proliferative phase than in the secretory.Conclusion1. In the present sudy, we first find that concentration of MIF in plasm... |
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