1.Proteome Analysis Of The Nasopharyngeal Carcinoma Cell Line (HNE1): Development Of A Membrane Protein Database 2.Proteome Analysis Of The Effect On Hepatic Stellate Cells Membrane With Herbal Compound 861

The plasma membrane proteins, as the "doorbells" and "doorways" of the cell, play crucial roles to the cell function. The proteomic definition of plasma membrane proteins is an important initial step in searching for novel tumor marker proteins expressed during the different stages of cancer progression.Nasopharyngeal carcinoma (NPC) is a commonly occurring tumor in southern China and south east Asia, occurring as one of the top ten cancers in frequency. This project was initiated with the purpose of separating and identifying the membrane protein of a human nasopharyngeal carcinoma cell line. We purified the crude plasma membrane and validated by electron microscopy observation. After two dimensional gel electrophoresis separation, silver staining, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and ESI-Q-TOF analyses, tryptic peptide masses were searched for matches in the SWISS-PROT, NCBI and Mascot databases. Over 196 spots were identified using this approach (including 92 known membrane proteins). These proteins include those involved in regulation of gene expression, affected the signal pathway, altered the cell metabolism, inhibit or accelerate cell proliferation, induce the cell apoptosis and intervened the tumor invasion. This study of the NPC proteome, coupled with similar proteome analyses of the whole cell, the normal nasopharyngeal tissues, and other nasopharyngeal carcinoma cell lines, defines the proteome of the NPC plasma membrane, which are valuable resources in studies on the differential protein expressions of human nasopharyngeal carcinoma.Herbal compound 861(Cpd861) is an effective compound in therapy against liver fibrosis and early hepatocirrhosis. To elucidate the antifibrotic mechanism of herbal compound 861, we employed proteomics techniques to study only the membrane proteins change in cultured rat hepatic stellate cells treated with Cpd861. Twenty five proteins thatchange in cultured rat hepatic stellate cells treated with Cpd861. Twenty five proteins that were up- and down-regulated were identified unambiguously, and the functional implications of 18 of these proteins are discussed in some detail.