Studies On The Immunal And Antioxidant Activities Of Laurencia Extract

Objective In this study we investigated the inhibition of various dosage of Laurencia extract(LET) from Sarcom 180(S180) in mice and the immune change,and observed its an-tioxidant activities .Method (1)The totel content of terpenoids in the LET was detected by HPLC and its mediallethaldose (LD50) was estimated by Horn assay. (2)The studies on the immunal activities of LET in mice vaccinated with S180 sarcom cells: 50 kunming mice were randomly divided into five groups . All mice were vaccinated with S180 sarcom cells in armpit of the left forearm and one day later four groups of them were respectively given LET(0,25,50, 100 mg/Kg wt) by stomach - perfusion. And the other group was the cytoxan positive group(20 mg/kg wt). Whole blood was collected 24 hours later after discontinuation of medication. The tumor, the thymus and the spleen were weighed exactly to calculate the tumor inhibition rate and the organ exponent. The proliferation effect of LET on spleen lymphocyte was evaluated by MTT assay. The contents of IgA,IgG,IgM in plasma were detected by the automatic biochemistry analyzer. (3)The studies on the antioxidant activities of LET in mice; 40 kunming mice were randomly divided into four groups. Each of them was given LET (0,25,50,100 mg/Kg wt) respectively for 20 days. Whole blood was collected 24 hours later after the last administration from the orbit. The oxidative DNA damaged of peripheral lymphocytes induced by H2O2 were analyzed by SCGE. The content of SOD,MDA ..GSH-Px in plasma were measured by biochemical methods.Results (1)The total content of terpenoids in the LET was proved to be 63. 29%. No toxicosis symptom was observed in all the animals for 14 days. No animal died. The LD50 was more than 3160mg/kg. The LET is low toxicity. (2)The studies on the immunal activities of LET in mice vaccinated with S180 sarcom cells; The average tumor weight of the supplemented group were all less than the control group (P<0. 05). The tumor inhibition rate of the high-dose group is 39. 1%. There were no differences between the supplemented groups and the cytoxan positive group(P>0. 05). The thymus exponent of the 25 mg/ kg wt LET group was higher than the control group and the cytoxan positive group(P< 0. 05). The spleen exponent of the 100 mg/kg wt LET group was higher than the control group and the cytoxan positive group (P<0. 05). The thymus exponent of the cytoxanpositive group was less than the control group(P<0. 05). The rate of the lymphocyte proliferation of the 25 mg/kg wt LET group and the 50 mg/kg wt LET group were all higher than the control group and the cytoxan positive group(P<0. 05). The rate of the lymphocyte proliferation of the 100 mg/kg wt LET group was significantly higher than the control group and the cytoxan positive group(P<0. 01). The rate of the lymphocyte proliferation had the tendency of increasing with the dosage elevation. The rates of the lymphocyte proliferation of the 100 mg/kg wt LET group was higher than that of the other supplemented groups(P<0. 05). The content of IgA in plasma of the100 mg/kg wt LET group was higher than the control group and the cytoxan positive group (P<0. 05). The contents of IgG and IgM of the 50 mg/kg wt LET group and the 100 mg/kg wt LET group were higher than the control group and the cytoxan positive group(P<0. 05). The content of IgA , IgG and IgM of the positive group was less than the control group(P<0. 05). (3)The studies on the antioxidant activities of LET in mice: There was a decreasing tendency of the oxidative DNA damage in peripheral lymphocytes induced by 5 mol/L, 10 mol/L and 20 mol/L H2O2. The oxidative damage on DNA induced by 10 mol/L H2O2 of the 50 mg/ kg wt LET group was less than that of the 100 mg/kg wt LET group and the control group(P<0. 05). The contents of MDA in the supplemented groups were less than the control group (P<0. 05). The contents of MDA and GSH-Px had no differences in four groups(P>0. 05).Conclusion (1)There are plentiful terpenoids in the LET. The LET has low toxicity. (2)The LET can inhibit the growt...