Expression Of PTTG And Its Relation With The Expression Of HPV16/18E6 And C-myc Protein In Cervical Carcinoma

Objective It has been suggested that pituitary tumor-transforming gene (PTTG), a novel oncogene, has played an important role in many human malignancies. However, little is known about its precise role in genesis and development of cervical cancer. In this study, the expression of PTTG protein were detected and the relationship with the clinical-pathological features, the expression of protein HPV16/18E6 and c-myc were analyzed in cervical cancer.Methods Expression of PTTG protein were assessed by immunohistochemistry method (SP method) respectively in 18 cases of CIN (Cervical intraepithelial neoplasia) (8 cases of CIN I, 10 cases of CIN II~III) and 49 case of invasive cervical carcinoma (38 cases of squamous carcinoma, 11 cases of adenous carcinoma) and compared with clinical characteristics, such as clinical-stage, pathological grade and lymphatic metastasis, and expression of protein HPV16/18E6 and c-myc. 15 case of normal cervical epithelia tissues were investigated as control group.Results 1. PTTG was expressed in cervical carcinoma tissues, the positive rate in CIN and invasive carcinoma were 44.44% and 63.27%. In contrast, normal cervical epithelium did not express PTTG. The expression of PTTG protein in cervical cancers was significantly higher than in normal cervical epithelia tissues (P<0.01). The expression of PTTG protein in CIN was also significantly higher than in normal cervical epithelia tissues (P<0.05).2. The positive rate of PTTG was strongly associated with the clinical-stage, histological grade and lymphatic metastasis. The expression rate of PTTG protein was stronger in stageIII and IV 87.51% than in stage I and II 51.52% (P0<.05). Similarly, the expression rate of PTTG protein was stronger in tissue with lymphatic metastasis 6/6 than those without lymphatic metastasis 42.31 % (P<0.05). The expression of PTTG protein was also correlated with histological grade (P<0.05). There was no relationship between PTTG protein expression and age or pathological type (P>0.05).3.The expression rate of PTTG protein in cervical cancer tissues with the HPV16/18E6 protein coexpression was higher than that in those without HPV16/18E6 protein coexpression (P<0.05).4. The PTTG protein expression in cervical cancers with the c-myc protein coexpression was higher than that in those without c-myc protein coexpressionConclusions 1 . The expression of PTTG protein was higher in cervical carcinomas than those normal tissues. The results suggest that the overexpression of PTTG may play an important role in the carcinogenesis of cervical carcinoma.2. Over expression of PTTG protein were related to clinical stage, histological grade and lymphatic metastasis in cervical carcinomas. The overexpression of PTTG may predict poor prognosis.3. High risk HPV infection maybe important in the development of cervical carcinoma. There was a relationship between PTTG expression and HPV16/18E6 or the expression of c-myc protion. They may play important roles in the carcinogenesis of cervical carcinoma.4. PTTG maybe a new target in the diagnosis and treatment of cervical carcinomas.Postgraduate Huang Aiping (Gynaecology)Directed by Prof. Wang Yankui...