Effect Of Surface Microtopography On Proliferation And Differentiation Of Osteoblasts

Objective: To study the influence of attachment , proliferation , differentiation of osteoblasts on different titanium surface microtopography in vitro. Materials and Methods: The third-fifth passage osteoblasts was poured onto all 3 different surface treatment discs-G (groove), SB (sandblasting), TPS (titanum-sprayed plasma) in 12-well plate, to study the influence of attachment, proliferation , differentiation of osteoblasts on different titanium surface microtopography by SEM observation , MTT Assay , Alkaline Phosphatase (ALP)activity and Osteocalcin(OC) measurements, plastic surface were used as a control. Results: (1)Cell Attachment: Osteoblasts adhered tightly to three different titanium surface, spreading in all directions; With G surface, osteoblasts were spindle-shaped with little cytoplasm; Osteoblasts were cubic, multangular and abundant cytoplasm on SB surface and ,TPS surface. (2)Cell Proliferation Assay:The number of cells are increasing from l-7days on all discs; while cell proliferation was significant higher on SB and TPS surface than G surface (P<0.05) and it was not significant difference between G and control surface (P>0.05). (3)ALP Measurement: An increas in ALP activity was detected on SB and TPS surface than control surface on 1, 5, l0days (P<0.05) ; At the same time, ALP activity increased significantly on TPS surface than on G surface (P<0.05); On 1, 3 , 5days, there was no significant difference of ALP activity between G surface and control surface (P>0.05) . (4)OC Measurement: An increase in OC production was detected on SB and TPS surface than control surface on 3, 5, lOdays (P<0.05) ; Synthesis of extracellular matrix proteins(OC) was more abundant on SB and TPS surface than G surface on 3, 5days (P<0.05) ; While OC production was not2statistically difference between SB and IPS surface (P>0.05) .Conclusion: The results indicate that rough surface is advantageous to attachment proliferation differentiation of osteoblasts than smooth surface, suggesting that rough surface might be inducing bone-formation early around dental implants in vivo.