| Objectives: In vitro fertilization-embryo transfer (IVF-ET) was a kind of assisted reproductive technology (ART) developed from 1970s. Its general process included controlled ovarian hyperstimulation (COH), oocyte retrieval, in vitro fertilization (IVF), embryo culture, embryo transfer, luetal phase support and et al. COH was to evoke multifollicular development under the controllable range through drug when IVF or intracytoplasmic sperm injection (ICSI). The objective was to obtain more high quality ovum and to increase the pregnancy rate. But the reason of luetal phase support after ET may be as followings: 1. High level of estradiol in follicular phase might shorten luteal phase. 2. Follicle suction or clusis made granulosa luteal cells lose. 3. GnRH-a restrained the function of hypophysis, and gonadotropic hormone excretion couldn't recover in short-term. Thus the progesterone supplement was necessary after ET. Ectogenesis gonadotropic hormone and sex hormone influenced the hormone balance inside the body directly or indirectly, which altered the environment of endometrium, confused the synchronism between endometrium and embryogenesis, and influenced the imbed and growth of the blastocyst.Epidermal growth factor (EGF) had been identified as an important growth factor recently. Lots of cells could excrete EGF inside the body except blastocyst trophoblastic cell, but on which existed epidermal growth factor receptor (EGFR). EGF combined EGFR on its target cells, which could accelerate placental cell to differentiate, proliferate, intake nutrition, enhance placental excretion function, and produce a significant effect on embryo and placent implantation.There was a complicated relation among gravida, placent and fetus. With the fast development of ART, the perinatal outcome of pregnancy after IVF was paid more and more attention. The aim of this study was to compare the expression of EGFR on placenta and reveal the change of placental morphology between the term pregnancy after IVF-ET and the normal term pregnancy, through which to provide theory basement and broaden a new idea for the perinatal outcome of pregnancy after IVF-ET.Methods: 1 Sample's source: 30 cases of term labour after IVF-ET and 30 cases of natural labour as control group with identical weeks of gestation were observed in this study. There was no statistical difference among age, height, body weigh, foetus number and delivery mod, and all the cases should be excluded pregnancy-induced hypertension syndrome, anemia, twins, triplets and other chronic diseases.2 Sample's collection and disposal: 2.1 After placent expulsed, take out 3 blocks of constitution immediately and randomly from intermediate zone and central zone of the floor plate of placental maternal surface in 4% glutaral fixation fluid; through fixation→desiccation→osmos→embed, slice with Leica ultramicrotome; double electron stain with acetic acid uranyl and lead nitrate; observe and take photographs under Hitachi-7500 transmission electron microscope.2.2 Simultaneously cut 3 blocks constitution vertically from placental maternal surface, after fixed in 4% neutrality formalin for 24~48 hours, embedded in paraffin, 5μm serial section, stained by hematoxylin-eosin (HE) and immunohistochemistry (IHC). 2.3 After shearing umbilical cord from its root, weigh the placent and neonate. 3 Detect the expression and distribution of EGFR on the placenta in each group through immunohistochemistry SP method. Quantitate the result of HE stain through the true color patholoty image analysis system. Observe the placental ultrastructure of each group under the electron microscope.Results: 1 The expression of EGFR was predominantly in syncy- tiotrophoblast cell's membrane and plasm, and the intensity of stain was stronger on brush border. Compared with the control group: the expression of EGFR in the group of IVF-ET was stronger, and the difference was significant (P<0.05).2 Compared with the control group, the number of blood vessels in the end... |
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