Relationship Between Activity Of T-PA And PAI In Gingival Crevicular Fluid And Periodontal Disease Activity

Objective: 1. To investigate the influence on the activity of tissue plasminogen activator (t-PA) in plasma sample with different disposal. 2. To investigate the activity of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) in gingival fluid from healthy, gingivitis and periodontitis patients. 3. To investigate the activity of t-PA and PAI before and after initial periodontal therapy in gingival fluid from chronic gingivitis and chronic periodontitis. 4. To investigate the relationship between activity of t-PA and PAI in gingival crevicular fluid and periodontal disease activity. Methods: 1.The elution efficiency of plasma t-PA activity were measured from three kinds of collection paper: whatman#1, periopaper, domestic filte paper. Periopapers soaked with plasma were divided into two groups. One group is treated elution beforehand, another group is treated no elution beforehand. Half samples of each group were stored at –20℃ and the rest were stored at –80℃ The activity of t-PA of each sample were measured in 1w, 2w, 4w, and 12w. 2. Gingival crevicular fluid were collected including 40 sites from 10 healthy , 44 sites from 11 gingivitis, 68 sites from 17 periodontitis. The activity of t-PA and PAI in gingival crevicular fluid were measured. 3. Seven gingivitis and thirteen periodontitis patients were selected and experienced initial periodontal therapy. Gingival crevicular fluid from 80 sites were collected before and after treatment, which includes 28 sites from Seven gingivitis, 52 sites from thirteen periodontitis. 4. 724 sites of 124 tooth from 5 chronic periodontitis patients were selected. Clinic index were collected at one month and four months after periodontal foundation treatment to find the active sites which`s new attachment loss ≥2mm between two visits. The activity of t-PA and PAI in gingival crevicular fluid were measured both in the active sites and the adjacent no-active sites. Results: 1. The results showed that periopaper has the highest elution efficiency of t-PA activity. And in the condition of no-elution beforehand, the activity of tissue plasminogen activator (t-PA) of the samples stored in –20℃ and –80℃ had no significant change within three months. 2. The results showed that significantly high activity of PAI in gingivitis and periodontitis sites , but there were no significant difference of t-PA activity among three group. The regression analysis show there to be significant correlations between PAI activity with clinic indexes such as BI(r=0.77, P<0.01),PI(r=0.67, P<0.05),PD(r=0.58, P<0.01),AL(r=0.45, P<0.01). 3. The results showed that there was no significant difference of the activity of t-PA before and after treatment in gingivitis but there was significant difference before and after treatment in periodontitis sites ; there was significant difference in PAI level before and after treatment both in gingivitis and periodontitis. The ratio of PAI/t-PA was significant differences before and after treatment in periodontitis, but not in gingivitis. 4. The estimation of periodontal disease activity per year may be 8.61% and the most common active sites were seen in maxillary molars. The results showed that no difference of the activity level of t-PA and PAI in active sites and adjacent no-active sites. Conclusions: 1. This study indicated that the periopaper is the ideal filter paper to collect GCF and the activity of tissue plasminogen activator (t-PA) can reserved at least three months in the condition of no elution beforehand from strip paper with –80℃. 2. This study indicated that the raise of PAI activity may restrain the activity of t-PA in GCF of gingivitis and periodontitis patients. The balance between t-PA and PAI activity in GCF need further research. 3. This study indicated the activity of PAI may be objective index to judge the inflammation of periodontium. The decrease of the ratio of PAI /t-PA in periodontitis may indicate the poor prognosi. 4. The present standard to diagnosis periodontal disease activity has it`s limite...