Skeletal Muscle Satellite Cell Transplanted Into Denervated Muscles To Retard The Progress Of Muscle Atrophy

Objective: To observe the effects of transplanted skeletal muscle satellite cell on denervated skeletal muscle atrophy in vivo. To study the effects of skeletal muscle satellite cell on muscle atrophy so as to provide the experimental data for treating denervated muscle atrophy. Method: Rat skeletal muscle satellite cells were obtained by the two-step method of collegenase-I and trypsin, the cells were purified by the Percoll density centrifugation and were subject to primary culture in vitro. Using frequent passaging culture obtain adequate single satellite cell. The cells were identified with cellular immunochemical stain, and preparation for satellite cells transplantation. Making use of 32 adult rats which were divided into 2 groups at random. There are 16 in control group and experimental group respectively. By cutting the right sciatic nerve of rats caused nerve despair about 1cm, so the animal model of denervated gastrocnemius muscles were formed. Being labeled with 4′,6—DIAMIDINO—2—PHENYLIN DOLE(DAPI) before muscle satellite cell transplanted. Muscle satellite cell and NS were implanted into denervated skeletal muscle respectively from the above 2 groups. Bilateral gastrocnemius muscles of each rat from the two groups were taken and weighed at second and eighth post-operative weeks respectively. The above muscles underwent anti-actin immunohistochemical staining and muscle cross-sectional area of fiber and the actin content of the rats were measured by the image analysis, the data of which was handled with SPSS software. Results: The satellite cells with fluorescence were observed in the skeletal muscle satellite cell transplant site. A portion of the transplanted cells had differentiated into the muscle fibers. The group of skeletal muscle satellite cells transplanted when two weeks denervated gastrocnemius muscle wet weight remnant rate(75.5823±7.3981)% and the denervated gastrocnemius muscle cross-sectional areas of fiber remnant rate(61.4280±17.3794)% and the denervated gastrocnemius muscle actin content(92.5492±12.6310) were better than in the control group(55.5359±11.5228)%,(35.4671±5.9619)%, (108.1428±9.9905)respectively. The group of skeletal muscle satellite cells transplanted when eighth weeks denervated gastrocnemius muscle wet weight remnant rate(45.0746±6.8860)% and the denervated gastrocnemius muscle cross-sectional areas of fiber remnant rate(43.2573±7.5996)% and the denervated gastrocnemius muscle actin content (109.0140±11.4526) were better than in the control group(26.8984±3.0435)%, (21.9359±5.6631)%, (120.2277±8.1153)respectively. Conclusion: The transplantation of muscle satellite cells into denervated muscles can alleviate denervated skeletal muscular atrophy and promote recovery of the muscular atrophy and muscular function after repaired nerve.