Clinical Significance Of β 2-glycoprotein I-dependent Antiphospholipid Antibodies In The Pregnancy Loss Patients

OBJECTIVE: Our purpose was to determine whether IgG isotype 2-glycoprotein I-dependent anticardiolipin antibodies and 2-glycoprotein I-dependent antiphophatidylserine antibodies may represent superior markers in pregnancy loss than do conventional antiphospholipid antibodies.STUDY DESIGN: The incidence of IgG isotype P 2-glycoprotein I-dependent and P 2-glycoprotein I-independent anticardiolipin antibodies and of anticardiolipin antibodies and of antiphophatidylserine antibodies was statistically compared between study groups with and without autoantibody-associated features of pregnancy loss. Sera from 88 women were collected from No.l Affiliated hospital, DaLian Medical University and DaLian Gynecology and Obstetrics hospital. They included sera from 59 patients with pregnancy loss such as natural abortion(one or two or more consecutive losses),and fetal death and 29 controls. Autoantibody levels by enzyme-linked immunosorbent assay for IgG isotype P 2-glycoprotein I-dependentand and P 2-glycoprotein I-independent anticardiolipin antibodies and anticardilpipin antibodies and antiphosphatidylserine antibodies were then compared.RESULTS:1.Natural abortion patients demonstrated a significantlyhigher incidence of glycoprotein I-dependent antiphophatidylserine antibodies than did controls (P0.05), fetal death patients demonstrated a significantly higher incidence of glycoprotein I-dependent anticardiolipin antibodies and anticardiolipin antibodies than did , controls(P<0.05). 2.Patients of three natural abortion groups noted a significantly higher incidence of glycoprotein I-dependent antiphophatidylserine antibodies than did controls (P<0.05), no different was between groups. 3. The sensitivity of anticardiolipin antibodies is higher than others (45.3%),the specificity of glycoprotein I-dependent anticardiolipin antibodies and glycoprotein I-dependent antiphophatidylserine antibodies is the highest (100.0%). 4. The cross-reactive positivity rate of 2-glycoprotein I-dependent anticardiolipin antibodies and 2-glycoprotein I-dependent antiphophatidylserine antibodies is the highest (33.3%), the one of 2-glycoprotein I-independent anticardiolipin antibodies and 2-glycoprotein I-dependent antiphophatidylserine antibodies is the lowest. 5.There is significant correlation between 2-glycoprotein I-dependent anticardiolipin antibodies and 2-glycoprotein I-dependent antiphophatidylserine antibodies ( R=0.673 , P0.01 ) ;anticardiolipin antibodies 2-glycoprotein I-dependent anticardiolipin antibodies and 2-glycoprotein I-independent anticardiolipin antibodies also correlate well (R=0.621 ;0.375,P<0.001). CONCLUSIONS:1. 2-glycoprotein I-dependent antiphophatidylserine antibodies is superior for the detection of natural abortion; 2-glycoprotein I-dependent anticardiolipin antibodies is superior for the detection of fetal death, They used together can improve the sensitivity of diagnosis.2. 3 2-glycoprotein I-dependent antiphophatidylserine antibodies is a marker for the detection of three natural abortion groups.3. The specificity of 2-glycoprotein I-dependent anticardiolipinantibodies is more higher than anticardiolipin , which are more accurate methods.