Studys On Prevalence Of Transfusion Transmitted Virus Infection And Sequence Of Transfusion Transmitted Virus In Xinjiang Intravenous Drug Users

Objective To investigate the prevalence of transfusion transmitted virus(TTV) infection and the sequence features of TTV among Intravenous drug users (IVDUs)of xinjing.Methods Anested polymerase chain reaction(nPCR) assay was established using two-set of primers designed from the first open read frame of TTV genome, TTV DNA was extracted . from 102 IVDUs and 38 normal persons, the frequent from 2059 to 2251 bp in the TTV genome was amplified and then cloned Into Easy Vector pUCm-T and then sequence analysis was performed . By ELISA the seroprevalence of viral markers of HCV and HBV infection was also examined.Results The IVDUs of 32.35%(33/102) were TTV DNA positive, but normal persons were 5.26%(2/38) positive. There was no relationship between TTV infection with nationalities, sex, age and persisted time of intravenous drug users . The sequence has a striking nucleotide homology to reported corresponding sites of two sequences AB008394(from Japan), AF079173 (from China)were 98%, the TTV strain is classified into genotype la. 68.75%, 7.84% were positive in anti-HCV, HBsAg in IVDUs. 8 of 33 cases with TTV infection for both anti-HCV and serological markers of HBV, 17 were positive for anti-HCV, 1 were positive for markers of HBVinfection .Conclusion The research proves that the infection of TTV and HCV is rather comprehensive in IVDUs of xinjiang . The IVDUs are high-danger crowd, amplified sequence of TTV from xinjiang had striking sequence homologies to reported twostrains(AB008394,AF079173)belong to the same genotype . TTV super-infection with hepatitis C and hepatitis B appeared to be associated with a same transmitted-route among IVDUs .