The Expression Of NF-Kappa B And ICAM-1 In Brain Of Rats During The Period Of Liver Ischemia-Reperfusion Injury

Objective: To observe the changes of NF-kappa B and ICAM-1 in the brain of rats during the liver ischemia-reperfusion injury, and explore whether the injury of the brain occurs during the event and the possible pathogenesis of the brain injury.Methods: A rat model of liver ischemia-reperfusion injury was established by imitation. 48 healthy rats were randomly divided into 6 groups as follows: the control group (the group of sham operation), simply ischemia 30 min without reperfusion (I group), reperfusion following ischemia 30 min (I/R group), 1 hr reperfusion following ischemia 30 min (I/R1h group). 2 hrs reperfusion following ischemia 30 min (I/R2h group) and 4 hrs reperfusion following ishcemia 30 min (I/R4h group). The changes of NF-kappa B and ICAM-1 in the forebrain cortex, hippocampine cortex and hypothalamus region measured by using immunohistochemical technique were compared among 6 groups.Results: During the process of liver ischemia-reperfusion. the levels of GPT. AKP and γ-GT in the serum, the expression of TNF- α and IL-1 β mRNA in the liver tissue significantly increased (p<0. 01), and theinflammatory infiltration of the neutrophilic granulocyte, cellular edema and necrosis in the liver were observed by HE staining. The brain cellular edema and necrosis was found in some rats of I/R2h and I/R4h group. Compared with the control group, the expression of NF-κB,ICAM-1 in the brain was significantly increased in I/R1h, I/R2h and I/R4h group(p<0.05 or <0.01), but not in I group and I/R group. No significant difference was found in the expression of NF-κB,ICAM-1 in the three regions of brain tissue(p>0.05).Conclusions: Brain injury occurred during the liver ischemia-reperfusion injury. NF-kappa B and ICAM-1 may be involved in the pathogenesis of the brain injury during the reperfusion following liver ischemia.