Experimental Study On BMSC Culture And Its Differentiation Towards Osteoblasts And Culture With Coral Materials

[Objective] To study state of culture and osteogenic differentiation of bone marrow stromal cells (BMSC) and observed the condition of BMSC's growth in the coral materials.[Methods] Obtained BMSC from the rabbit about 2 months then cultured the cells and induced differentiation toward osteoblasts with special cultural fluid in vitro. The induced BMSC were harvested and then seeded into the coral. Observed the condition of both cells and coral under an inverted phase contrast microscope, scanning electron microscope (SEM) and electron probe microscope analysis (EPMA). [Results] (1) BMSC grew by static adherence, differentiated to osteoblasts and had strong capacity of proliferation, the shape of cells was fusiform or polygon, like osteoblasts. And ability of formation of calcifying nodules was detected. Alkaline phosphatase (ALP) activity of BMSC increased significantly by Gomori method. The level of osteocalcin was higher than control group greatly. Amount of Calcium ion and Phosphor ion was more than control group remarkably, when scanned with Electron probe microscope analysis. (2) When subcultured at about 12th, whatever induced BMSC or BMSC in complete culture medium changed in shape. And its transformed entirely at 16th or so, its' shape became bigger and flatter than before, and adhere to bottom of bottle irregularly. Its' activity decreased and tended to decrepitude. (3) BMSC might grow, differentiated and proliferated in and on the coral materials normally. Some BMSC entered the hole of coral, some overlayed the hole under the SEM.[Conclusions] (1) To obtain the BMSC from bone is convenient and cells are sufficient with a little trauma and complication in this experiment. BMSC have a strong activity of proliferation and osteogenic capacity. So BMSC are apt to serve asseed cells for bone tissue engineering. (2) Coral has a good cellular compatibility without cyto toxic and tumorigenicity and BMSC may grow, proliferate and form mineralization area on the coral surface or in the holes. Coral is a favorable bone tissue engineering scaffold.