| Objective : Hind limbs ischemia/ reperfusion is a commonly occurring event in clinical practice,such as major vascular surgery with aortic cross-clamping ,peripheral arterial occlusion and reperfusion, limb replantation and so on . Reperfusion of limbs always initiates both local and systematic damage. Sometimes it can alsodevelop into multiple organ dysfunction (MOD), which is the most serious complications following hind limbs ischemia- reperfusion, and this syndrome presents a high mortality rate in patient. Up until now the mechanism of this phenomenon yet clear, many study has indicated that oxygen free radicals playing an important role in the physiopathological of ischemia-reperfusion injury. It not only initiates lipid peroxidation process but also lead to inflammation responds and tissue injury. Despite advances in medical knowledge and technology, little progress has been made in the treatment of such patients. Propofol is a new an intravenous anesthetic, which has gained worldwide application not only as an anesthetic agent but also as a sedative agent in the intensive care unit because it can be easily titrated and offers the prospect of rapid recovery. It has been shown to exhibit signigicant antioxidant activity and resembles the endogenous antioxidant α-tocopherol(vitamin E) by reacting with free radicals to form a phenoxyl radical in vitro. So Propofol have obvious protective action on organs. In this study, we used propofol on the model of distant organs injury following hind limbs ischemia/ reperfusion in rats to observe the changes of MDA content, SOD activity, and the expression of inducible nitric oxide syntheses (iNOS), intercellular adhesion molecule type 1(ICAM-1) in lung, liver, kidney tissue and combined with the changes of pathology in lung, liver, kidney tissue to discuss the organ protective effects of propofol and its mechanism. We hope this study will offer the experiment support for the application of propofol on organ protection in clinical practice.Methods: Thirty-six healthy wistar rats weighing 300~350g were randomly assigned into three groups and anesthetized by 3% pentobarbital 30mg﹒kg-1 i.p. : (1) sham group(n=12), the rats received the operation without other treatment; (2) hind limbs ischemia/ reperfusion group (I/R group, n=12), hind limbs 4-hour ischemia followed by 6-hour reperfusion; 3)propofol treated group (I/R+P group, n=12), the rats received a bolus of 0.5% propofol 5mg﹒kg-1 iv and then continuously infused 10mg﹒kg-1﹒h-1 10 minute before reperfusion. The I/R group were administered with an equivalent dosage of normal saline (NS) alone instead of propofol, that is 1ml·kg-1 bolus followed by 2ml·kg-1·h-1 continuously. At the end of the experiment, all rats were killed by carotid bloodletting, the lung, liver and kidney tissue was sampled to determined the MDA content, SOD activity, the water content of lung, the expression of iNOS, ICAM-1 and have microscopic examination (light and electron microscopy).ResultThe variety of ?MDA in every tissue or organ as follow:The MDA content of kidney(0.769±0.224 nmol/mg prot)and that of liver(0.699± 0.213 nmol/mg prot)in group I/R?were significantly larger than those (0.616±0.139 nmol/mg prot, 0.481±0.134 nmol/mg prot by turns) in the group sham(P<0.05).The MDA level of lung in group I/R(1.33±0.473 nmol/mg prot)were notably higher than that in group sham(0.847±0.15 nmol/mg prot)(P<0.01). Compared with the group I/R,the MDA of kidney(0.599±0.122 nmol/mg prot)and that of liver MDA( 0.502 ±0.2 nmol/mg prot) decreased obviously in group I/R+P(P<0.05)and ?also did that of lung MDA(0.813±0.273 nmol/mg prot)(P<0.01). After compared I/R+P group with sham group, we found that their content of MDA in these three tissues had no difference by statistis analysis(P>0.05). 2 The alteration of SOD activity in every tissue The SOD activity of lung was 11.871±1.821 NU/mgprot in sham group. It was 7.415±2.315 NU/mg prot in group I/R(remarkably lower than group sha... |
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