Adoptive Transfer Of Primed IEL Induces Intestinal Mucosal Immune Responses And Protects Against Murine Infection With Toxoplasma Gondii

The propagation of Toxoplasmosis is mainly though mucosa and placenta, and is second only malaria and schistosomiasis. The parasite infects man and animals, and there is no effective medicine to treat it. Therefore immune prophylactic would be highly valuable. Recent years, the adoptive transfer of effector cells was made to treat cancer, virus and bacterium diseases. This study was performed to determine the mucosal response and protection against challenge in mice following IEL adoptive transfer that separated from the mice after oral infection with Toxoplasma gondii at the specific times.Six- to seven-week-old 72 BALB/c mice were used, half of them were female. 36 mice donor provided IEL after oral infected 5×104 tachyzoites or received no tachyzoites, the 36 recipient mice were divided into experimental groups (IEL7, IEL9, IEL11, IEL13 and IEL15 group ) and control group, in each group six mice, receiving the primed and unprimed 3×105 IEL via tail vein, respectivily. The primed IEL were purified on days 7, 9, 11, 13 and 15 postinfection (p.i.) and the unprimed IEL obtained from naive mice. The mice were challenged with 5×104 tachyzoites on day 4 following the adoptive transfer of IEL. On the day 13 post-challenge, the tachyzoites in brain, lung and spleen were purified and counted. The T lymphocyte subsets in Peyer's patches were determined and the intestinal IgA antibodies were detected.The tachyzoites burden in brain, lung and spleen were decreased 81.13%, 58.43%, 70.97% after the primed IEL adoptive transfer. The tachyzoites burden in the mice received the primed IEL purified on day13 p.i. were decreaced most remarkably. The protective ability of IEL is dependent on the time of p.i. The primed IEL purified on days13 p.i. provided most protection for the mice. The percentage of CD4+ and CD8+ T subsets, CD4+/ CD8+ ratio in Peyer's patches and IgA level of intestines in experimental groups were higher than control group. These results suggest that immune protection provided by primed IEL were higher than that provided by unprimed IEL and that the primed IEL adoptive transfer can up-regulate the mucosa immune response in Peyer's patches and level of IgA antibody and induce protective mucosal cell and antibody response.