Cationic Liposomes Complexed To Plasmids Encoding Endostatin And/or Angiostatin Inhibit Lewis Lung Cancer In Mice Model

Objective: To investigate the inhibitory effect of cationic Liposomes complexed to plasmids encoding endostatin and/or angiostatin on the growth and metastasis of Lewis lung cancer implanted in mice model. Methods: C57BL/6j mice were inoculated subcutaneously with Lewis lung cancer cell suspend liquid, cationic liposome complexed plamids encoding angiostatin and endostatin were administrated intratumorally to inhibit the growth and metastasis of the implanted tumor. the size change of the tumor; the activity,nourishment, survival period of the mice; metastasis in lung were observed to evaluate the function of cationic liposome complexed plamids.Result: 1. tumor in the control group growed continually,tumor in the treatment group growed slowly after third day ,and contracted after a week, the mice treated with pAng( 69±10mm3)or pEnd (38±8mm3)or pEnd and pEnd (45±10mm3) comparing with empty control (4736±275mm3)or SA-liposome control(6134±235mm3)showed significance in tumor size on eighteenth day(p<0.01). 2. the activity,nourishment, et al of the mice in all treatment group had no obvious changes, however that of the two control were bad gradually. 3.the number of the metastasis in lung treated with pang(3.0±1.4) pEnd,pEnd and pEnd no metastasis comparing with empty control (18.7±11.3)or SA-liposome control(17.2±4.8) showed significance(p<0.05). 4. the average survival period of all treatment group(above 50d) was longer than that of empty control(28.2 ± 4.1d) and SA-liposome control(31.2 ± 3.8d)(p<0.01). Conclusion: Administrate intratumorally cationic liposome complexed plamids encoding angiostatin and endostatin can powerfully inhibit the growth and metastasis of implanted Lewis lung cancer in mice model.