Studies On Inducing Apoptosis Effects Of Agaricus Blazei Murill Extract For MGC-803 Gastric Cancer Cell Lines

Objective: To investigate the anti-proliferation and inducing apoptosis of the Agaricus Blazei Murill extract for MGC-803 gastric cancer cell line in vitro. Methods: Using the MTT assay and colonogenic assay , the inhibition of cell' s growth of the Agaricus Blazei Murill extract for MGC-803 gastric cancer cells was examined; using HE stain and acridine orange(AO)/ethidium bromide(EB) fluorescence stain, inducing apoptosis of Agaricus Blazei Murill extract for MGC-803 gastric cancer cells was examined. The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry (S-P method) before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro.Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light. It was showed by using acridine orange (AO) / ethidium bromide (EB) fluorescence staining that MGC-803 gastric cancer cells after treated by the Agaricus Blazei Murill extract were not only necrosis but also apoptosis, but the apoptosis was as the dominant way. The bcl-2 and bax of relating-apoptosis genes protein was significantly changed before and after treating with the Agaricus Blazei Murill extract (P<0.001) . Conclusions: The Agaricus Blazei Murill extract has significant inhibition ofproliferation and inducing apoptosis for MGC-803 gastric cancer cells in vitro and its possible mechanism of inducing apoptosis for tumor cells might be related with the expression changes of the bcl-2 and bax of relating-apoptosis genes protein. This experiment results will provide theoretical basis for clinical applying and further research of the Agaricus Blazei Murill extract.