The Study On Molecular Servomechanism Of CD35,CD44 And CD62P On Thrombocyte And Erythrocyte In Tumor Hemomctastasis

Invasion and metastasis are the most remarkable biological signs of malignanttumor, which are also the most important lethal factors in clinic.Hemometastasis ofcarcinoma need not only evading of immunological surveillance but alsocounteracting bloodstream shear stress to localize, invade and grow in normal tissue.The original mechanism of malignant tumor metastasis has not been radicallyclarified to this day. The newly decade researches had substantiated that thrombocyteand its adhesion molecules exert most important effect to tumor hemometastasis.Capture of tumor cells contradicting bloodstream shear stress is the premise conditionfor hemometastasis.Tumor cell seized could interact with thrombocytes andleukocytes by some adhesion molecules and medium in blood,then coming into beingexiguous cancer embolus and adhering to vascular endothelial cells of metastatictissue, consequently inducing tumor cell to traverse through vascular endothelium intotissue and to localize,invade and proliferate,which completed metastasisprocess,however thrombocyte is the most important key to thrombosis and theinteraction of tumor cell with thrombocyte included a lot of procedures and variableadhesion molecules. Guo Feng et al. for the first time found that most cancer cell strain can activatecomplement by sideway and sequentially adhere to C3b.Qian Baohua et al.'s study alsodemonstrated that thrombocyte membrane express vast of CD35 molecules(complement receptor type I, CR1), which conjectured in theory that CD35 could leadnatural immune response between thrombocyte and tumor cell, and that maybe CD35is a key approach to interaction of thrombocyte with tumor cell. Clinicalinvestigations made clear that majority of cancer patients' blood was in highconglutinated state, which is correlated tightly with thrombocyte activation degree.CD62P molecule (P-selectin) is the symbol of platelet activation that can enhanceefficiency of platelet adhesion to cancer cell, moreover its crisis for rolling and - 6 -第二军医大学硕士学位论文 英文摘要invading of carcinoma in vessels. Qian Baohua et al. confirmed that CD35 and CD44expression on red cells in cancer patients decreased significantly than that of normalcontrol group, and so on red cell natural immune response to cancer cell withdecreasing ability to inhibit carcinoma metastasis which indicated that CD35 andCD44 molecules on erythrocyte exert inhibition effect on carcinoma metastasis.Thrombocyte and erythrocyte perhaps operate different immunological response tocarcinoma. This study established the method of platelet immunological adhesion to cancercell S180 and researched on the discrepancy of ability to adhere to S180 betweencancer patients and normal group to discuss its meaning for interaction of plateletwith cancer cell; using flow cytometry to mensurate CD35,CD44 and CD62Pexpression on platelet and red cell in cancer patients and normal group, investigatedthe expression discrepancy and correlation variance and discussed the biologicalimportance of these data for carcinoma hemometastasis. The study providedbrand-new experimental evidence for inhibiting carcinoma hemometastasis andcreative idea of prevention against cancer metastasis.PartⅠ Foundation and clinical rudimentary application of platelet immunological adhesion to tumor cell S180 methodObjective To found method of platelet immunological adhesion to tumor cell S180 and to contrast the discrepancy of ability to adhere to S180 with ACD anticoagulant or EDTA anticoagulant, and the difference of ability with cancer patients or normal group, and to discuss the biological significance of these difference for carcinoma hemometastasis and its clinical rudimentary application value.Methods To incubate S180 cell suspension with PRP(platelet rich plasma) in water...