Study On Association Of TNFα Gene Polymorohism With Graves Disease

Objective :Study on associations between tumour necrosis factor (TNF)agene polymorphisms and Graves disease(GD) susceptibility, to investigate the mechanisms and etiology of GD.Subject and Method: We select 100 cases of Shandong Chinese patients with GD and 100 cases health control conducting health examniantion all without consgenuity from QILU hospital afflitaed to Shandong university . GD group were divided into three subgroups acording to sex. with or without GD family history and with or without Thyroid- associated ophthalmopathy( TAO) . Genomic DNA were extracted from peripheral white blood cell, Alleles and genotypes were determined by polymerase chain reaction and sequence specific primers. The frequencies distrubition of allele and genotype of two groups were tested by Hard-Weinberg test to domenstrate their populative representation, the comparison of numeration data between two groups were tested by chi square. P<0.05 was considered significant.Results: 1.There have no significant difference comparing allele and genotype frequencies of TNFα +488 > -308 between GD and control group.2.There have no significant difference comparing allele and genotype frequencies of TNFα+488 > -308 between male and female , with and without GD family history or with and without TAO GD subgroups.3.There have no significant difference comparing allele and genotypefrequencies of TNFα +488, -308 between different servity TAO subgroups.Conclution:1 There are G/A polymorphisims in TNFα +488 , -308 in ShanDong Chinese. The allele A frequencies in health control is adout 12% in +488 and 20% in-308.2 There are no association between TNFα +488, -308 polymorphisims and GD susceptibility.3 There are no association between TNFα +488, -308 polymorphisims and male or female GD.4 There are no association between TNFα +488, -308 polymorphisims and GD with or without GD family history.5 There are no association between TNFα +488, -308 polymorphisims and TAO.