Study On Value Of PP65 Detection Of Human Cytomegalovirus Antigenaemia In Progressive Stroke

Objective:Progressive stroke is one of the most common and special kinds in cerebral thrombosis. The symptoms of this disease continue progressing with routine therapy, so both the mortality and the deformity of it are high. It is also a difficult problem for clinical doctors to investigate some satisfactory treatment. Arteriosclerosis is regareded as the major pathology basis of cerebral infarction, but clinical research finds some patients with arteriosclerosis have not traditional danger factors. It is important for clinical researchers to find new related factors and develop treatments. Recently, most researchers pay more attention to infection, especially the relationship between cytomegalovirus and arteriosclerosis. But there are not reports about study on the relationship between cytomegalovirus and progressive stroke until now. This study tries applying the immunity fluorescence method to determine human cytomegalovirus PP65 in Progressive stroke and Constant infarction, investigating the relationship of active infection of human cytomegalovirus and Progressive stroke. Through active monitoring of human cytomegalovirus PP65 antigenaemia and experimental antiviral therapy, we discuss the rules of human cytomegalovirus changing from positive to negative in Progressive stroke and assess the value of human cytomegalovirus PP65 antigenaemia detection in Progressive stroke in order to provide a new clue for clinical doctors to prevent and cure Progressive stroke early. Methods:Immunofluorescence was used to HCMV-PP65 antigenaemia detection in peripheral WBC of 223 patients, including 90 patients with Progressive stroke and 133 patients with Constant infarction. We compared the positive rates of antigen with the two groups and give antiviral drug (Phosphonoformic Acid) treat to fifteen Progressive stroke patients with positive PP65 antigenaemia detection. HCMV-PP65 antigen was checked at weekly intervals. After it turned to negative we would continue to give antiviral drug treat for another three to five days in order not to reoccur. Results:In Progressive stroke and Constant infarction groups: the positive cases of HCMV-PP65 antigenaemia were twenty and sixteen, and the positive rates of it are 22.22% and 12.03%. The positive rate of Progressive stroke is obviously higher than that of Constant infarction. This prompts the relationship between Progressive strokeand active HCMV infection is closer. The condition of fifteen patients with Progressive stroke whose HCMV-PP65 antigenaemia detection positive were aggravate after given conventional treat. But when they were given antiviral therapy, the condition of thirteen stop progressing in two to five days and turn to better in ten to twenty seven days. HCMV-PP65 antigenaemia turn to negative and the time of them concentrates to two to three weeks. When HCMV-PP65 antigenaemia turn from positive to negative, we would continue to treat for another three to five days and find no one reoccur. There was one case whose result was no good and there was one case died. Conclusions: 1 HCMV-PP65 antigenaemia detection can diagnose active infection of HCMV early and quickly; 2 Active infection of HCMV may be associated with Progressive stroke; 3 HCMV-PP65 antigenaemia detection can guide antiviral drug treatment and evaluate curative effect of it;...