Studies On The Bioactivity Constituents Of Evodia Lepta (Spreng.) Merr.

Evodia lepta (spreng.) merr. is mainly distributed in South China. The aerial part of Evodia lepta (spreng.) merr. is used as a Chinese folk medicine to treat trauma, abscesses, wound infection , eczema, dermatitis and haemorrhoids.In order to find out bioactive constituents, from ethanolic extract of this plant we isolated ten compounds. On the basis of physical and chemical properties as well as detail spectroscopic analysis (UV, IR, MS, ¹HNMR, ¹³CNMR, DEPT, HMQC, HMBC), these compounds have been identified as psoralen( â…  ), dictamnine( â…¡ ), evolitrine( â…¢), kukosaginine( â…£ ), skimmianine(â…¤), 2' ,3' -dehydromarmesin(â…¥), cerotinic acid(â…¦), β -sitosterol(â…§), 7-oxo-β-sitosterol (â…¨) and pachypodol (â…©). All these compounds were obtained from this plant for the first time. Compound psoralen(â… ), 2' ,3' -dehydromarmesin (â…¥), 7-oxo-β -sitosterol (â…¨) and cerotinic acid(â…¦) were new to the genus.To analyze the constituents of Evodia lepta leaves, the essential oil was extracted by water distillation method and analyzed by gas chromatography-mass spectrometry for the first time. As a result, fourty-six constituents were separated. Among which, the chemical structures of eighteen constituents were identified, and their relative content were determined. The sum of their contents was 91. 04% of the oil. The main constituents in the essential oils from Evodia lepta leaves are hexadecanoic acid(30.74%), dibutyl phthalate(15.87%), phytol(13.46%), dioctyl phthalate(7.58%), hexahydropseudoionone(6.37%), and phthalic acid-diundecyl ester(3.85%)In order to investigate the antioxidant activity of Evodia lepta. , the effects of pachypodol and 2' ,3' -dehydromarmesin from Evodia lepta on the rat cereberal cortex cells injuried by H₂O₂ in vitro were evaluated.