The Expression And Significance Of High-risk HPV And P53 Associated Gene Protein In Cervical Squamous Cell Carcinoma: Amplication Of Tissue Microarray Techinique

Objective:To study the expression and significance of HPV 16/18 . 31 / 33 DNA nd P5 3 , p63 , P 16IK4A , P21 wAFl.and MDM2 proteins in invasive squamous ell carcinoma of cervix(ISCC).To investigate the correlation of HPV 16 / 18 , 31 / 33 DNA and proteins referred to above and the relationship between the iomarkers and clinical pathological parameters.To analyse the significance of he biomarkers in the occurrence and development of ISCC SO as to provide the aluable evidence for the early clinical diagnosis and genetic therapy of ISCC . Method : 120 cases of ISCC were embedded in 240 spots tissue microarrays.In Situ Hybridization(ISH)was used to detect the expression ofHPVl6/ 18 , 31 / 33 DNA and the SP immunohistochemical method was performed to research the expression ofp53,P16INK4A,P21wAF1,P63 and MDM2 protein in ISCC,CIN and NCE.Results was assessed by SPSS vision 12.5. Results:1,The positive expression rate ofHPVl6/18 in ISCC was markedly higher than in CIN and NCE(P<0.05).No correlation was found between HPV 16/18 and clinical pathological parameters. 2.There iS no abvious difference about HPV3 1/33 expression in ISCC , C1N and NSE(P>O . 05) . but we found the significant difference between HPV3 1/33 and lymph node transferation. 3 . The positive expression rate of P53 protein was 50 % in ISCC , which was significantly higher than in the CIN and NSE . The positive rates of P5 3 protein expression were significantly higher in the cases of histological grade III than the cases of histological grade I-II and the cases with lymph node metastasis than those without lymph node metastasis(P<0.01) 4.We found the difference of the expresslon of P63 between ISCC and CIN,ISCC and NCE(P<0.05).The positive rates of P63 were significantly higherin the cases of pathological grade III than those in grade I—II. 5 . The positive expression rate of P 16^INK4A had the significant difference between ISCC and CIN,ISCC and NCE.Significant relationship was observed between P 16INK4A protein expression and differentiated grades of the ISCC. 6 . The difference of P2 1 wAF1 protein was significant among ISCC . C1N . and NCE(P<0 . 01) . The positive expression rate of P21WAF1 protein in the cases of deeper invasion was significantly higher than those in the cases of superficial invasion(PINK4A and P21^WAF1 protein,but a negative correlation with MDM2 protein. Conclusion:1.There was a higher positive expression ofHPVl6/18 than that of HPV3 1/33 in ISCC and we found that mtp53 protein expression rate was very high because HPV DAN was positive in ISCC.The results of our study indicate that the positive expression rate of P 16INK4A,P21 wAF1 was high , but not low in ISCC . The overexpression of MDM2 proteinsuggested that MDM2 gene might play a important role as an oncogene on the occurrence in ISCC.But the positive expression of P 16^INK4A.P21^wAF1hint that P 16INK4A and P21 wAF1 genes might not act as a suppressor—gene only on the occurrence and development in ISCC.The overexpression of P63 protein in ISCC suggested that p63 gene might be oncogene but not suppressor — gene on the occurence and development in ISCC...